User:Paul Rothenberg/Notebook/Pauls Notebook/2013/02/25: Difference between revisions
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* | *Procedure: | ||
*1. 50x TAE stock buffer was diluted to 1x TAE buffer, using 2 mL of 50x buffer and 98 mL of ultra-pure water (1) | *1. 50x TAE stock buffer was diluted to 1x TAE buffer, using 2 mL of 50x buffer and 98 mL of ultra-pure water (1) | ||
*2. 0.25 grams of agarose was dissolved in 25 mL of TAE | *2. 0.25 grams of agarose was dissolved in 25 mL of TAE | ||
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*4. The TAE and agarose was poured into the electrophoresis chamber and left to harden; electrophoresis buffer was added following | *4. The TAE and agarose was poured into the electrophoresis chamber and left to harden; electrophoresis buffer was added following | ||
*5. 5 microliters of DNA and 1 microliter of dye; 6 microliters of the DNA ladder were added to the wells | *5. 5 microliters of DNA and 1 microliter of dye; 6 microliters of the DNA ladder were added to the wells | ||
*6. The gel was run for | *6. The gel was run for 55 minutes at 90V | ||
*7. The gel was then placed in Ethidium Bromide rinse and shaken for 15 minutes | |||
*8. The gel was removed and placed in a TAE rinse and shaken for another 15 minutes | |||
*9. The gel was exposed in an electrophoresis chamber | |||
*Data: | |||
*1. No experimental DNA bands were visible under UV radiation. The gel will either have to be stained in ethidium bromide for a longer period of time, or a new gel will have to be rerun. | |||
Revision as of 11:54, 25 February 2013
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25 February 2013
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