User:Paul Rothenberg/Notebook/Pauls Notebook/2013/02/11

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11 February 2013

  • Procedure:
  • 1. 10 milligrams of solidified P-sorbitol and P-Mannitol in Phosphate buffer was measured six times and placed into 6 tubes
  • 2. 1 mL of each solvent was placed in each tube under the fume hood (see solvents below
  • 3. The tubes were each placed on a vortex for approximately 2 minutes to try to dissolve the sorbitol
  • 4. 5 of the 6 tubes (water not included - the sorbitol dissolved with no issue) were placed in a sonicating water bath


  • Data for P-Sorbitol in Phosphate buffer (pH 7):
  • 10.0 mg in distilled water
  • 10.1 mg in acetone
  • 9.4 mg in methanol
  • 10.0 mg in chloroform
  • 10.8 mg in acetonitrile
  • 12.2 mg in ethyl acetate


  • Data for P-Mannitol in Tris Buffer (pH 9) (No solvents added today)
  • 11.6 mg in
  • 10,5 mg in
  • 11.7 mg in
  • 10.0 mg in
  • 10.5 mg in
  • 11.7 mg in


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