Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2013/02/04"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/02/04 Entry for User:Paul_Rothenberg/Notebook/Pauls_Notebook)
 
(Entry title)
Line 6: Line 6:
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
+
==4 February 2013==
* Insert content here...
+
* Objectives:
 +
 
 +
*1. Run UV-VIS and Fluorescence on protein dissolved in Chloroform in Acetate buffer and water in acetate buffer (See Dhea Patel's Notebook - Insert link)
 +
*2. Use a Lyopholizer to solidify proteins dissolved in solution
 +
 
 +
 
 +
*Lyopholizer Procedure:
 +
 
 +
*1. The Lyopholizer was turned on and let cool to -50 Centigrade.
 +
*2. The proteins were solidified in liquid nitrogen, and placed in the vacuum
 +
 
 +
*Fluorescence and UV-VIS Procedure:
 +
 
 +
*1. Chloroform and water samples were run in each spectroscope
 +
2*
 +
 
 +
 
 +
*10 mg of Sorbitol, Hemoglobin, and PEG previously solidifed was added to 5 out of 6 tubes for solvent to be added (See Dhea's Notebook)
 +
 
 +
 
 +
 
 +
*Data:
 +
 
 +
*
  
  

Revision as of 11:07, 4 February 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

4 February 2013

  • Objectives:
  • 1. Run UV-VIS and Fluorescence on protein dissolved in Chloroform in Acetate buffer and water in acetate buffer (See Dhea Patel's Notebook - Insert link)
  • 2. Use a Lyopholizer to solidify proteins dissolved in solution


  • Lyopholizer Procedure:
  • 1. The Lyopholizer was turned on and let cool to -50 Centigrade.
  • 2. The proteins were solidified in liquid nitrogen, and placed in the vacuum
  • Fluorescence and UV-VIS Procedure:
  • 1. Chloroform and water samples were run in each spectroscope

2*


  • 10 mg of Sorbitol, Hemoglobin, and PEG previously solidifed was added to 5 out of 6 tubes for solvent to be added (See Dhea's Notebook)


  • Data: