Difference between revisions of "User:Nkuldell/SAGA swap pt 2"

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including biobrick ends for expression of ORF in S. cerevisiae
 
including biobrick ends for expression of ORF in S. cerevisiae
 
This subunit is homolog of S. cerevisiae SGF73 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf73]] subunit of SAGA and deletion of this subunit in S. cerevisiae results in viable cells but no induction of PAU genes under hypoxic conditions.
 
This subunit is homolog of S. cerevisiae SGF73 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf73]] subunit of SAGA and deletion of this subunit in S. cerevisiae results in viable cells but no induction of PAU genes under hypoxic conditions.
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alignment of S. cerevisiae Sgf73p with S. pombe SPCC126.04c shows alignments btw S.cerevisiae aa 68 to aa 398 with S. pombe seq aa 74 to 318. Sgf73 has C-term region without homology to smaller S.pombe protein.
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[[Image:Sc Sp Sgf73.pdf]]and [[Image:Sp Sc Sgf73pseq.pdf]]

Revision as of 12:53, 5 July 2006

Reagents

BBa_Y00073 [[1]]
had DNA 2.0 codon-optimize S. pombe gene SPCC126.04c [[2]] including biobrick ends for expression of ORF in S. cerevisiae This subunit is homolog of S. cerevisiae SGF73 [[3]] subunit of SAGA and deletion of this subunit in S. cerevisiae results in viable cells but no induction of PAU genes under hypoxic conditions.

alignment of S. cerevisiae Sgf73p with S. pombe SPCC126.04c shows alignments btw S.cerevisiae aa 68 to aa 398 with S. pombe seq aa 74 to 318. Sgf73 has C-term region without homology to smaller S.pombe protein. File:Sc Sp Sgf73.pdfand File:Sp Sc Sgf73pseq.pdf