User:Nikolai Eroshenko

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"All there is to thinking, is seeing something noticeable which makes you see something you weren’t noticing, which makes you see something that isn’t even visible." -Norman Maclean


  • gene synthesis
  • genome engineering
  • protein design
  • molecular biology methods development
  • plant-based antibody and vaccine production


Gene assembly from chip-synthesized oligonucleotides
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George_Church, Current Protocols in Chemical Biology 4 (2012)

Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy AOP (2011)

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George_Church, Nature Biotechnology 28:1295 (2010) cover

Role of bioinspired polymers in determination of pluripotent stem cell fate
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)


G4 Harvard SEAS Bioengineering Church Lab (HMS Genetics, NRB 238/Wyss Institute)


Feng Zhang's lab - McGovern Institute/Broad Institute, MIT
Kit Parker's lab - Harvard Bioengineering
Raj Rao's lab - VCU Chemical Engineering
Curtis Taylor's lab - VCU Mechanical Engineering (now at UF)