Difference between revisions of "User:Nikolai Eroshenko"

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Orthogonal amplification and assembly of nucleic acid sequences PCT/US2011/057075 [http://patentscope.wipo.int/search/en/detail.jsf?docId=WO2012154201&recNum=228&docAn=US2011057075&queryString=EN_ALLTXT:(quantum)&maxRec=55226 pending]
Orthogonal amplification and assembly of nucleic acid sequences, PCT/US2011/057075, [http://patentscope.wipo.int/search/en/detail.jsf?docId=WO2012154201&recNum=228&docAn=US2011057075&queryString=EN_ALLTXT:(quantum)&maxRec=55226 pending]

Revision as of 10:10, 7 February 2013


I am building new technologies for basic and applied biology. Long-term research interests include:

  • human transgene vectors that integrate genes into precise locations on the genome
  • cheaper, faster, and more accurate gene synthesis
  • library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions


Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)

Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy 19:469 (2012)

Gene assembly from chip-synthesized oligonucleotides (PDF)

Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4:1 (2012)

  • Gene Assembly by Subpool PCR (GASP) oligo design scripts and server

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips (PDF)

Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)

Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF)

Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)


Orthogonal amplification and assembly of nucleic acid sequences, PCT/US2011/057075, pending


George Church (2009-now)

Wyss Institute (2009-2011)

Raj Rao (2006-2008)

Curtis Taylor (2006-2007)


Harvard University (2008-now) PhD candidate, Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering


eroshenkona AT gmail

Nikolai Eroshenko
77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115