User:Nathan H. Kipniss/Notebook/20.109 Final Project: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 3: | Line 3: | ||
==Background== | ==Background== | ||
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes. | In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes. | ||
[[Media:S12_M2D7_TR-Orange.txt]] | |[[Media:S12_M2D7_TR-Orange.txt]] | ||
==Ideas== | ==Ideas== | ||
Revision as of 18:55, 30 April 2012
Elucidating the function of D24H Inverse Pericam mutant
Background
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes. |Media:S12_M2D7_TR-Orange.txt
Ideas
-working under the assumption that the SDM did indeed work (sequencing with BLAST, discontinuous mega-blast suggests it did indeed work)
- consider looking at calmodulin only.
