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Personal notes on Kohler Illumination
This topic was already discussed on the entry of 09/15/2010. See the following link: Kohler Illumanation
In the section Set up for Kohler Illumination, a number of steps are listed. I will just add a couple of notes in some of them.
This is done first by placing the objective at the correct distance from the sample slid. If the refractive index is greater than 1, a good amount of a thick oil needs to be placed between the objective and the sample slide.
Then, center the slid by adjusting the knobs that move the slide left or right and away or toward the observer.
This is the most difficult part of the process. Therefore, it would be best to follow these upcoming instructions to get better results.
Bring the condenser down, closer to the sample.
If the intensity of light has changed, then play with the condenser’s focusing knobs until you find a bright spot.
If the intensity of the light has not changed, close the diaphragm a little more until the view gets dimmer. Then play with the condenser’s focusing knobs until you find a bright spot.
Repeat the process until the image of the edge of the diaphragm is best focused.
This is a very tedious process. It requires a lot of practice and tolerance. So be patient.