Difference between revisions of "User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/02/12"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/02/12 Entry for User:NYOUSHA_Yousefi/Notebook/Protein_Engineering)
 
(Procedure)
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Entry title==
 
==Entry title==
* Insert content here...
+
Tris buffer preparation
  
 +
==Objective==
 +
Two buffer solutions of 10mM Tris+50mM NaCl and 10mM Tris+1M NaCl at Ph of 8.5 were prepared
 +
 +
==Procedure==
 +
# 1.12g Tris and 2.9g NaCl were added to 1L of distilled water to prepare 10mM Tris+50mM NaCl solution and 1.12g Tris and 58.4g NaCl were added to 1L of distilled water to prepare 10mM Tris+1M NaCl solution
 +
# Ph meter was calibrated with the standard pH solutions (pH= 4, 7, 10) and afterwards pH of these two Tris buffer solutions were adjusted to 8.5 by adding concentrated 1M HCl
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 16:45, 18 February 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Tris buffer preparation

Objective

Two buffer solutions of 10mM Tris+50mM NaCl and 10mM Tris+1M NaCl at Ph of 8.5 were prepared

Procedure

  1. 1.12g Tris and 2.9g NaCl were added to 1L of distilled water to prepare 10mM Tris+50mM NaCl solution and 1.12g Tris and 58.4g NaCl were added to 1L of distilled water to prepare 10mM Tris+1M NaCl solution
  2. Ph meter was calibrated with the standard pH solutions (pH= 4, 7, 10) and afterwards pH of these two Tris buffer solutions were adjusted to 8.5 by adding concentrated 1M HCl