Difference between revisions of "User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/02/05"

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(Procedure)
(Objective)
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==Objective==
 
==Objective==
The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml Tris pH 8
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The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml phosphate pH 7
  
 
==Procedure 1==  
 
==Procedure 1==  

Revision as of 17:20, 18 February 2013

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Title

Hb buffer exchange

Objective

The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml phosphate pH 7

Procedure 1

  1. column chromatogrophy was rinsed with 25ml of distilled water and 25ml of new buffer solution containing 0.5g PEG, 1g manitol,and 50ml of phosphate buffer, pH 8
  2. 2.5ml of WT Hb and 2.5ml of the new buffer were added to the column and this 5ml was collected in a new tube (2% manitol, 1% PEG, WT Hb, pH 7 phosphate)

Procedure 2