Difference between revisions of "User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/02/05"

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(Objective)
(Procedure)
Line 12: Line 12:
 
The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml Tris pH 8
 
The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml Tris pH 8
  
==Procedure==  
+
==Procedure 1==  
 +
# column chromatogrophy was rinsed with 25ml of distilled water and 25ml of new buffer solution containing 0.5g PEG, 1g manitol,and 50ml of phosphate buffer, pH 8
 +
# 2.5ml of WT Hb and 2.5ml of the new buffer were added to the column and this 5ml was collected in a new tube (2% manitol, 1% PEG, WT Hb, pH 7 phosphate)
 +
 
 +
==Procedure 2==
 +
 
  
 
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Title

Hb buffer exchange

Objective

The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml Tris pH 8

Procedure 1

  1. column chromatogrophy was rinsed with 25ml of distilled water and 25ml of new buffer solution containing 0.5g PEG, 1g manitol,and 50ml of phosphate buffer, pH 8
  2. 2.5ml of WT Hb and 2.5ml of the new buffer were added to the column and this 5ml was collected in a new tube (2% manitol, 1% PEG, WT Hb, pH 7 phosphate)

Procedure 2