User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/02/05: Difference between revisions
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==Procedure 2== | ==Procedure 2== | ||
# The bacteria expressing Mn and Ni were allowed to grow in media for 6 hours after addition of IPTG, which is a signal that stimulates protein expression | |||
# The media was divided and balanced into four smaller flasks and they were placed in centrifuge at 4500rpm, 4 degree C for 20 min | |||
# After 20 min, the supernatant of the samples was discarded and the pellets including WT Ni and Mn Hb, BL-21(DE3) were resuspended in Tris pH 9 buffer | |||
# These samples were stored at -80 degree C freezer | |||
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Revision as of 17:29, 18 February 2013
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TitleHb buffer exchange ObjectiveThe wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml phosphate pH 7 Procedure 1
Objective 2Extracting bacteria cells Procedure 2
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