User:Moira M. Esson/Notebook/CHEM-581/2013/04/03: Difference between revisions
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==DSC== | ==DSC== | ||
*Please refer to Karlena Brown's laboratory notebook [[User: | *Please refer to Karlena Brown's laboratory notebook [[User:Karlena_L._Brown/Notebook/PVOH_Research/2013/04/03|2013/04/03]] for any information concerning DSC sample preparation. | ||
* For many of the DSC samples run, the microsphere samples analyzed were not completely dry. As such, the DSC peak analysis will be affected by the presence of safflower oil encompassed in the sample | |||
* X-ray diffraction will be run on all prepared microsphere samples in order to determine whether clay has agglomerated separately from the PVOH | |||
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'''DSC samples''': | |||
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Name''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample Mass (mg)''' | |||
| align="center" style="background:#f0f0f0;"|'''Pan / Lid Mass (mg)''' | |||
|- | |||
| 50:50 PVOH 130K NaMT||3.12||50.01 | |||
|- | |||
| 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR||2.29||50.17 | |||
|- | |||
| 90:10 PVOH 130K Laponite||2.59||50.12 | |||
|- | |||
| 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR||2.27||50.28 | |||
|- | |||
| 90:10 PVOH 146K 110% CEC NaMT w/ DMHXLBR||2.65||50.11 | |||
|- | |||
| 50:50 PVOH 146K 110% CEC NaMT w/ DMHXLBR||2.50||50.17 | |||
|} | |||
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'''DSC Data Collection''' | |||
Figure 1. DSC graph of 50% CEC NaMT clay with Bu<sub>3</sub>HdP<sup>+</sup> surfactant | |||
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[[Image:50% CEC NAMT.jpg|400px]] | |||
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Figure 2. DSC graph of 110% CEC Laponite clay with DMHXLBr surfactant | |||
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[[Image:110 LP DMHXLBR.png|500px]] | |||
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Figure 3. DSC graph of 110% CEC NaMT clay with DMHXLBr surfactant | |||
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[[Image:110 NaMT DMHXLBR.png|500px]] | |||
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Figure 4. DSC graph of Laponite clay | |||
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[[Image:Laponite.png|500px]] | |||
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Figure 5. DSC graph of NaMT clay | |||
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[[Image:NaMT.png|500px]] | |||
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Figure 6. DSC graph of microspheres prepared with a 90:10 ratio of PVA MW 146,000-186,000:Laponite clay | |||
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[[Image:90-10 146K LP.png|500px]] | |||
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==Diffusion Testing== | ==Diffusion Testing== | ||
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# The microspheres were removed from their respective vial and placed in new, clean 20mL vials. | # The microspheres were removed from their respective vial and placed in new, clean 20mL vials. | ||
# 15mL of deionized H<sub>2</sub>O were added to each vial. | # 15mL of deionized H<sub>2</sub>O were added to each vial. | ||
# | # A timer was started, and every 15 minutes, a sample of distilled H<sub>2</sub>O was removed from the beaker and placed in an unfrosted cuvette. | ||
# After running the sample on the fluorimeter, the sample was readded to the diffusion vial. | |||
# This was repeated for 2 hours. | # This process was repeated for 2 hours. | ||
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'''General information on the parameters of the fluorescence run''': | '''General information on the parameters of the fluorescence run''': | ||
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#Scan rate/Speed: 1200 | #Scan rate/Speed: 1200 | ||
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'''Spectra''' | |||
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Figure 7. Diffusion Testing of Rhodamine 6G in microspheres prepared with 90:10 ratio of PVA MW 146,000-186,000: 50% CEC NaMT | |||
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[[Image:90 pva 146 50NaMT micros diffusion.jpg]] | |||
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*Fluorescence intensity maximum is 6.985. | |||
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Figure 8. Diffusion Testing of Rhodamine 6G in microspheres prepared with 90:10 ratio of PVA MW 146,000-186,000: 110% Laponite | |||
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[[Image:90 PVA 146 110LP micros diff.jpg]] | |||
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*Fluorescence intensity maximum is 5.109. | |||
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Figure 9. Diffusion Testing of Rhodamine 6G in microspheres prepared with a 90:10 ratio PVA 146,000-186,000: NaMT | |||
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[[Image:90 PVA 146 NaMT micros diff test.png]] | |||
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*Fluorescence intensity maximum is 26.734 | |||
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Figure 10. Diffusion Testing of Rhodamine 6G in microspheres prepared with a 50:50 ratio PVA 146,000-186,000: NaMT | |||
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[[Image:50 PVA 146 NaMT micros diff.png]] | |||
*Fluorescence intensity maximum is 16.350 | |||
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Figure 11. Diffusion Testing of Rhodamine 6G in microspheres prepared with a 50:50 ratio PVA 146,000-186,000: 50% CEC NaMT | |||
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[[Image:50 PVA 146 50NaMT micros diff.png]] | |||
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*Fluorescence intensity maximum is 11.661 | |||
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Figure 12. Diffusion Testing of Rhodamine 6G in microspheres prepared with a 50:50 ratio PVA 146,000-186,000: 110% CEC Laponite | |||
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[[Image:50 PVA 146 110 LP.png]] | |||
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*Fluorescence intensity maximum is 137.319. | |||
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==Notes== | |||
* The tested "microspheres" more closely resemble smaller, more circular, more flexible hydrogels. Other microsphere samples were prepared and may more closely resemble microspheres. | |||
* The microsphere prepared with a 50:50 ratio PVA 146,000-186,000: 110% CEC Laponite caused the most diffusion of dye. All microspheres will be used for pressure testing, however, because not all dye leaked out. | |||
* All curves will be integrated in order to determine the concentration of dye leaked. | |||
Revision as of 06:58, 15 April 2013
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Objectives
DecantingThe general protocol described on 2013/03/01 was followed.
DSC
Diffusion TestingA new general protocol for the preparation of was used for the diffusion testing of the prepared microspheres. It was determined that the previous protocol did not allow for a quantitative understanding of the amount of dye diffused from the samples.
Notes
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