Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-581/2013/03/20"

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==Microsphere Dye Addition==
 
==Microsphere Dye Addition==
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*The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] was followed.
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*An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
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<br>
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Dilution calculations:
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'''1μM Rhodamine 6G Dye Concentration (90:10)'''
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  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
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  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
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'''1μM Rhodamine 6G Dye Concentration (50:50)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
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<br>
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Dye Added to the Microspheres:
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
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| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
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|-
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| 50:50||110% CEC NaMT||0.05010||0.05100||40||165||61
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|-
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| 90:10||110% CEC NaMT||0.01070||0.09100||40||92||109
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|}
  
  

Revision as of 17:34, 24 March 2013

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Objectives

  1. Finish rinsing and filtering prepared microspheres with hexanes.
  2. Continue microsphere preparation.
  3. Add dye to prepared microspheres in preparation for diffusion testing on microspheres with dye additive.


Microsphere Dye Addition

  • The general protocol for dye addition to PVA hydrogel/microspheres described on 2013/02/20 was followed.
  • An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels


Dilution calculations: 1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL


Dye Added to the Microspheres:

PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 110% CEC NaMT 0.05010 0.05100 40 165 61
90:10 110% CEC NaMT 0.01070 0.09100 40 92 109



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