User:Moira M. Esson/Notebook/CHEM-581/2013/03/01

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Objectives

  1. Conduct pressure tests on all hydrogels using an unmodified pipette.
  2. Run fluorescence on all samples collected from the pressure tests.
  3. Decant the safflower oil from the microsphere samples prepared on 2013/02/22.


Pressure tests using an unmodified pipette

  • All pressure tests were conducted following the general protocol described on 2013/02/20.
  • Pressure tests were conducted using an unmodified pipette in order to determine if the very slight amount of sheer pressure caused by an unmodified pipette would cause a detectable amount of dye diffusion.


Table 1. Information concerning the pressure testing involving hydrogels with an unmodified pipette.

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
1 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1006
2 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1022
3 90:10 130K Laponite 0.1031
4 50:50 146K 110% CEC Laponite w/ DMHXLBR 0.1066
5 90:10 130K 110% CEC Laponite w/ DMHXLBR 0.1083
6 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1003
7 50:50 130K 110% CEC Laponite w/ DMHXLBR 0.1036
8 50:50 146K Laponite 0.1016
9 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011
10 90:10 146K 110% CEC NaMT w/ DMHXLBR 0.1020
11 50:50 130K NaMT 0.1024
12 50:50 130K 50% CEC NaMT w/ Bu3HdP+ 0.1015
13 50:50 146K 110% CEC NaMT w/ DMHXLBR 0.0998
14 90:10 130K 50% CEC NaMT w/ Bu3HdP+ 0.1026


 Figure 1. Fluorescence of hydrogel samples modified with Lamponite clay using an unmodified pipette for pressure testing


 Figure 2. Fluorescence of hydrogel samples modified with NaMT clay using an unmodified pipette for pressure testing


Fluorescence of Rhodamine 6G collected after pressure test of hydrogels with NaMT clay and unmodified pipette.png

 Figure 3. Fluorescence of all hydrogel samples using and unmodified pipette for pressure testing


Notes:

  • The amount of dye, or concentration of dye, that was diffused from the hydrogels using an unmodified pipette was significantly lower than that of a bent pipette.
  • The general trends observed using a bent pipette were also observed with an unmodified pipette.


Microspheres

  • After the freeze