User:Moira M. Esson/Notebook/CHEM-581/2013/03/01

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  1. Conduct pressure tests on all hydrogels using an unmodified pipette.
  2. Run fluorescence on all samples collected from the pressure tests.
  3. Decant the safflower oil from the microsphere samples prepared on 2013/02/22.


Pressure tests using an unmodified pipette

  • All pressure tests were conducted following the general protocol described on 2013/02/20.
  • Pressure tests were conducted using an unmodified pipette in order to determine if the very slight amount of sheer pressure caused by an unmodified pipette would cause a detectable amount of dye diffusion.


Figure 1. Fluorescence of hydrogel samples modified with Lamponite clay using an unmodified pipette for pressure testing Figure 2. Fluorescence of hydrogel samples modified with NaMT clay using an unmodified pipette for pressure testing Figure 3. Fluorescence of all hydrogel samples using and unmodified pipette for pressure testing
Notes:

  • The amount of dye, or concentration of dye, that was diffused from the hydrogels using an unmodified pipette was significantly lower than that of a bent pipette.
  • The general trends observed using a bent pipette were also observed with an unmodified pipette.


Microspheres

  • After the freeze