User:Moira M. Esson/Notebook/CHEM-581/2013/02/20: Difference between revisions
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==Hydrogel Pressure testing== | ==Hydrogel Pressure testing== | ||
* After performing diffusion tests on all prepared hydrogels on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/15|2013/02/15]], it was determined that the hydrogels prepared with DMSO and dye added prior to the freeze thaw method did not leak dye at a fast rate. As such, pressure tests will be performed using these hydrogels because all collected dye will be caused solely by sheer pressure. | |||
*First, the most effective pipette for pressure testing was determined. | |||
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General Protocol: | |||
# A 9 in. Corning disposable, non-sterile Pasteur pipette was heated using a Bunsen burner and was bent in various ways(multiple bends at the stem of the pipette and the top, bends only at the top, etc.). Note: Pictures of bent pipettes will be uploaded for reference. | |||
# Using a razor blade, the hydrogel for testing was cut into small cubes. The mass of each tested sample should be approximately 0.1g. | |||
# 3mL of distilled H<sub>2</sub>O were added to the pipette and using a ribber bulb squeezed out of the pipette. The 3mL of H<sub>2</sub>O were collected in a beaker. | |||
# Fluorescence was then run on the collected sample. | |||
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*The same hydrogel sample will be used to test the various Pasteur pipettes. As such, the sample with the largest amount of fluorescence will indicate the most effective manner in which to bend the Pasteur pipette to create the most sheer pressure. | |||
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Pressure samples: | |||
Revision as of 11:21, 23 February 2013
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Objectives
Microsphere preparation
Hydrogel Pressure testing
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