User:Moira M. Esson/Notebook/CHEM-581/2013/02/15: Difference between revisions
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# The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker. | # The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker. | ||
# The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres. | # The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres. | ||
# The blender was turned on a low setting for | # The blender was turned on a low setting for 7 minutes. | ||
# The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added. | # The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added. | ||
# The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | # The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. |
Revision as of 17:29, 18 February 2013
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Objectives
Microsphere Preparation
General Protocol:
For a 90:10 ratio: (25mL)(1μM)/(92μM)=0.272mL For a 50:50 ratio: (25mL)(1μM)/(165μM)=0.152mL
Fluorescence
General Protocol:
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