User:Moira M. Esson/Notebook/CHEM-581/2013/02/15: Difference between revisions
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# The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker. | # The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker. | ||
# The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres. | # The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres. | ||
# The blender was turned on a low setting for | # The blender was turned on a low setting for 7 minutes. | ||
# The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added. | # The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added. | ||
# The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | # The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | ||
# Repeat this freeze-thaw cycle three times. | # Repeat this freeze-thaw cycle three times. | ||
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This procedure was adapted from [http://www.sciencedirect.com/science/article/pii/S0168365998000893] | |||
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Preparation of Microspheres: | Preparation of Microspheres: | ||
*Information about prepared microspheres: | *Information about prepared microspheres: | ||
{| {{table}} | {| {{table}} | ||
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| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
|- | |- | ||
| Composition of hydrogel(ratio of PVA to clay)||Amount of PVA added(g)||Amount of clay added(g)|| | | Composition of hydrogel(ratio of PVA to clay)||Amount of PVA added(g)||Amount of clay added(g)||Volume H<sub>2</sub>O(mL)||Amount of mineral oil added(mL) | ||
|- | |- | ||
| 90:10 PVA:110% NaMT||0.9099||0.1045|| | | 90:10 PVA:110% NaMT||0.9099||0.1045||29||28 | ||
|- | |- | ||
| 50:50 PVA:110% NaMT||0.5076||0.4913|| | | 50:50 PVA:110% NaMT||0.5076||0.4913||27||26 | ||
|- | |- | ||
| 90:10 PVA:110% Lamponite||0.9010||0.0998|| | | 90:10 PVA:110% Lamponite||0.9010||0.0998||26||25 | ||
|} | |} | ||
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==Fluorescence== | ==Fluorescence== | ||
*The six hydrogel samples that were allowed to soak in Rhodamine 6G were tested for the rate of diffusion of Rhodamine 6G from the samples. | *The six hydrogel samples that were allowed to soak in Rhodamine 6G were tested for the rate of diffusion of Rhodamine 6G from the samples. | ||
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Spectra: | Spectra: | ||
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Figure 1. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel | |||
[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]] | |||
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'''Correction''': The x-axis should be labeled Wavelength(nm). The image will be corrected. | |||
Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel | |||
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[[Image:2 hr diffusion test 50 MW 146 NaMT.png]] | |||
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Figure 3. Diffusion test fluorescence spectra for 90:10 ratio of PVA MW 146,000-186,000:Lamponite | |||
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[[Image:2 hr diffusion test 90 MW 146 LP.png]] | |||
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Figure 4. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:NaMT | |||
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[[Image:2hr diffusion test 90 MW 146 NaMT.png]] | |||
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Figure 5. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:50% NaMT | |||
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[[Image:2hr rhodamine diffusion test 90 MW 146 50% NaMT.png]] | |||
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*'''Correction: The x-axis should be labeled Wavelength(nm). The image will be corrected''' | |||
Observations: | Observations: | ||
*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method. | *Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method. | ||
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*Comparing the 50:50 ratio of PVA:clay and the 90:10 ratio, the hydrogels with 50:50 ratio had more dye leak out of the hydrogel than the 90:10 ratio. Perhaps indicates a more effective pressure stimuli. | *Comparing the 50:50 ratio of PVA:clay and the 90:10 ratio, the hydrogels with 50:50 ratio had more dye leak out of the hydrogel than the 90:10 ratio. Perhaps indicates a more effective pressure stimuli. | ||
*In the future, when performing the diffusion tests, after taking a sample every fifteen minutes, the sample will be readded to the test beaker rather than discarded. | *In the future, when performing the diffusion tests, after taking a sample every fifteen minutes, the sample will be readded to the test beaker rather than discarded. | ||
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Revision as of 07:43, 5 April 2013
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Objectives
Microsphere Preparation
General Protocol:
Fluorescence
General Protocol:
Observations:
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