User:Moira M. Esson/Notebook/CHEM-581/2013/02/08: Difference between revisions
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==Objectives== | ==Objectives== | ||
# Prepare another dye stock solution for PVA-clay microspheres to soak it. | # Prepare another dye stock solution for PVA-clay microspheres to soak it. | ||
# Allow the PVA-clay microspheres to absorb dye. | # Allow the PVA-clay microspheres to absorb dye. | ||
# Remove soaking hydrogels from water and allow then to absorb dye. | # Remove soaking hydrogels from water and allow then to absorb dye. | ||
# Synthesize more PVA-clay microspheres. | # Synthesize more PVA-clay microspheres. | ||
# Finish preparing PVA MW 130,000 hydrogels | |||
<br> | <br> | ||
==Dye solution preparation== | ==Dye solution preparation== | ||
*As with the preparation of the PVA-clay hydrogels, Rhodamine 6G will be used as the dye. An internal concentration of 1μM dye will be used. | *As with the preparation of the PVA-clay hydrogels, Rhodamine 6G will be used as the dye. An internal concentration of 1μM dye will be used. | ||
**Because of the removal of all liquid from the microspheres, a 1μM stock solution will be prepared. A | **Because of the removal of all liquid from the microspheres, a 1μM stock solution will be prepared. A dilution was used from the previously prepared stock solutions. After allowing the the microspheres to absorb the dye for an extended period of time, the microspheres will be rinsed with hot and cold water to remove any DMSO. | ||
'''Calculations''' | '''Calculations''' | ||
M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | ||
V<sub>1</sub>=(1μMx25000μL)/(92μM)=271.74μL | V<sub>1</sub>=(1μMx25000μL)/(92μM)=271.74μL | ||
*The use of 25mL will make it so a dilution is not necessary for each microsphere dye soak. The prepared 1μM solution | *The use of 25mL will make it so a dilution is not necessary for each microsphere dye soak. The prepared 1μM solution was prepared in a volumetric flask, parafilmed, and stored for subsequent use. | ||
<br> | |||
==Microsphere preparation== | |||
*The general protocol described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/06|2013/02/06]] was followed. | |||
==Hydrogel Preparation== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Prepared PVA MW 130,000 hydrogels''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Composition||Amount of PVA(g)||Amount of clay added(g)||Concentration of dye solution added(uM)||Volume of dye solution added(mL) | |||
|- | |||
| 50:50 PVA:110% Lamp||0.4998||0.4998||165||0.0364 | |||
|- | |||
| 50:50 PVA:110% NaMT exchange||0.4992||0.4991||165||0.0364 | |||
|} | |||
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|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 14:01, 23 February 2013
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Objectives
Dye solution preparation
Calculations M1V1=M2V2 V1=(1μMx25000μL)/(92μM)=271.74μL
Microsphere preparation
Hydrogel Preparation
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