User:Moira M. Esson/Notebook/CHEM-581/2012/11/16: Difference between revisions
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#Using forceps, carefully place the cut strip of film in a clean quartz cuvette. | #Using forceps, carefully place the cut strip of film in a clean quartz cuvette. | ||
#Using a spatula, gently press the film against the cuvette. | #Using a spatula, gently press the film against the cuvette. | ||
#Carefully place the cuvette into the UV-vis. | #Carefully place the cuvette into the Shimadzu 2550 spectrophotometer. | ||
# | #Measure following the general protocol for UV-vis measurements | ||
<br> | |||
Porphyrin film UV-vis pathlengths: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Film measured''' | |||
| align="center" style="background:#f0f0f0;"|'''Thickness of film(mm)''' | |||
|- | |||
| 2.4394g PVOH(22K)+0.5mLGA+0.99mL porphyrin prepared 10/19||0.77 | |||
|- | |||
| PVOH(22K) 1.0073+1.0mL GA+1mL porphyrin prepared 10/24||0.79 | |||
|- | |||
| PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin prepared 10/26||1.01 | |||
|- | |||
| PVOH(22K) 1.0067g+1mL GA+1mL porphyrin||1.27 | |||
|- | |||
| PVOH(22K) 1.05225g+ 0.5mL GA+1mL porphyrin prepared 10/24||0.8 | |||
|} | |||
==Standardization of sodium bicarbonate== | |||
New weights of PHP used: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Trial number''' | |||
| align="center" style="background:#f0f0f0;"|'''Mass PHP(g)''' | |||
|- | |||
| 1||1.13465 | |||
|- | |||
| 2||1.13423 | |||
|- | |||
| 3||1.13518 | |||
|} | |||
Titration information: | |||
*The general protocol for titrations was used. The sodium bicarbonate solution prepared[[User:Moira_M._Esson/Notebook/CHEM-581/2012/11/09|2012/11/09]] was used as the base. Three drops of bromothymol blue was added each PHP solution. | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Trial number''' | |||
| align="center" style="background:#f0f0f0;"|'''Initial Volume (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final volume (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total volume used(mL)''' | |||
|- | |||
| 1||2.3||10.2||7.9 | |||
|- | |||
| 2||10.2||19.2||9 | |||
|- | |||
| 3||19.2||29||9.8 | |||
|} | |||
==Swelling for GA films== | |||
General Protocol for film swelling: | |||
#Cut small piece of crosslinked glutaraldehyde films and place in small beaker. Allow to completely dry for ~48 hours | |||
#Weigh small piece of film. Record mass. | |||
#Place dry piece of film in 10mL beaker and add enough deionized H<sub>2</sub>O to completely cover the film. Parafilm the beaker and allow to sit for ~48 hours. | |||
#Reweigh the film. Record the mass. | |||
<br> | |||
Please refer to [[User:Melissa_Novy/Notebook/CHEM-581/2012/11/16|Melissa Novy's lab notebook]] for swelling information. | |||
*Note: Small pieces of film used for FTIR measurement were used for swelling information. | |||
==Color Change of porphyrin films== | |||
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Revision as of 16:20, 26 November 2012
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Objectives
UV-vis porhyrin filmsGeneral protocol for UV-vis on porphyrin films:
Standardization of sodium bicarbonateNew weights of PHP used:
Titration information:
Swelling for GA filmsGeneral Protocol for film swelling:
Color Change of porphyrin films |