Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-581/2012/10/19"

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==Crosslinking==
 
==Crosslinking==
*The general protocol for crosslinking glutaraldehyde films described on
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*The general protocol for crosslinking glutaraldehyde films described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/14|2012/09/14]] was followed.
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*For the films containing porphyrin, the exact amount of H<sub>2</sub>SO<sub>4</sub> fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis
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Revision as of 07:12, 26 October 2012

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Objectives

  1. Finish running AA
  2. Crosslink porphyrin films
  3. Reprepare Fe3O4 filtrate that was prepared 2012/09/26

AA

  • Standard solutions were reprepared by Melissa Novy. Please refer to Melissa Novy's lab notebook for calculations and preparation

AA Data collected:

' Concentration [ppm] Abs. [ ] BG '
0.01 -0.00600 0.00200
0.05 -0.01160 0.00540
1 -0.00920 0.00150
2 0.11710 0.00470
3 0.20330 0.00330
Given Conc. [ppm] Abs. [ ] BG Conc. [ppm]
1 min NaMT-CuSO4 7.4 0.37690 0.00430 5.63990
10 0.47600 0.00620 7.01910
5 min NaMT-CuSO4 7.4 0.38080 0.00350 5.69420
10 0.48150 0.00600 7.09560
10 min NaMT-CuSO4 7.4 0.29540 0.00370 4.50580
10 0.37640 0.00480 5.63300
1 min 100%CEC NaMT-CuSO4 7.4 0.48030 0.00680 7.07890
10 0.61880 0.00950 9.00630
5 min 100%CEC NaMT-CuSO4 7.4 0.46120 0.00640 6.81310
10 0.62080 0.00790 9.03420
10 min 100%CEC NaMT-CuSO4 7.4 0.43300 0.00400 6.42070
10 0.62960 0.00480 9.15660
1 min 50%CEC NaMT-CuSO4 7.4 0.47470 0.00530 7.00100
10 0.62050 0.00490 9.03000
5 min 50%CEC NaMT-CuSO4 7.4 0.46110 0.00310 6.81170
10 0.60940 0.00580 8.87550
10 min 50%CEC NaMT-CuSO4 7.4 0.46800 0.00390 6.90770
10 0.59620 0.00440 8.69180

Crosslinking

  • The general protocol for crosslinking glutaraldehyde films described on 2012/09/14 was followed.
  • For the films containing porphyrin, the exact amount of H2SO4 fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis