Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2014/04/16"

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#DSC
 
#DSC
 
#Microscope
 
#Microscope
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<br>
 +
==Microscope==
 +
*Please refer to Puja Mody's notebook for all microscope data
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<br>
 +
==Atomic Absorption==
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*AA was used to analyze the presence of both iron and gold for all remaining samples
 +
<br>
 +
Figure 1. Calibration curve for iron standards
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[[Image:Calibration curve of gold standards for atomic absorption analysis of biomineralized proteins with gold and magnetite nanoparticles correct .png|500px]]
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<br>
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Figure 2. Calibration curve for gold standards
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[[Image:Calibration curve of gold standards for atomic absorption analysis of biomineralized proteins with gold and magnetite nanoparticles correct.png|500px]]
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<br>
 +
Table 1. Iron Atomic Absorption Analysis of samples
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample name'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Iron in sample (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Total concentration Iron originally in solution (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Iron incorporated (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Percent Iron incorporated (%)'''
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|-
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| 13500:1 Mag:BSA N2 prep||0.022256152||1765.35||1765.327744||99.99873928
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|-
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| 13500:1 Mag:BSA N2 prep with Au||0.022219961||1765.35||1765.32778||99.99874133
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|-
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| 13000:1 Mag:BSA oven||0.02224606||1765.35||1765.327754||99.99873985
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|-
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| 16500:1 Mag:Heme oven||0.02224084||1765.35||1765.327759||99.99874015
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|-
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| 16500:1 Mag:Heme:Au oven||0.022238231||1765.35||1765.327762||99.99874029
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|-
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| 16500:1 Mag:Heme:Au sonicated||0.022220831||1765.35||1765.327779||99.99874128
 +
|-
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| 13500:1 Mag:BSA:Au sonicated||0.022207781||1765.35||1765.327792||99.99874202
 +
|-
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| 13000:1 Mag:BSA:Au oven||0.022180812||1765.35||1765.327819||99.99874355
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|}
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<br>
 +
Table 2.Gold atomic absorption analysis of samples
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Sample name'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Gold in sample (ug/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Total concentration Gold originally in solution (ug/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration gold incorporated (ug/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Percent Gold incorporated (%)'''
 +
|-
 +
| 13500:1 Mag:BSA N2 prep with Au||0.04467344||49.2425||49.19782656||99.90927869
 +
|-
 +
| 13500:1 Mag:BSA:Au sonicated||0.0464768||49.2425||49.1960232||99.90561649
 +
|-
 +
| 13000:1 Mag:BSA:Au oven||0.04685952||49.2425||49.19564048||99.90483928
 +
|-
 +
| 16500:1 Mag:Heme:Au oven||0.04447792||49.2425||49.19802208||99.90967575
 +
|-
 +
| 16500:1 Mag:Heme:Au sonicated||0.04400784||49.2425||49.19849216||99.91063037
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|}
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<br>
  
 
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Objectives

  1. AA
  2. DSC
  3. Microscope


Microscope

  • Please refer to Puja Mody's notebook for all microscope data


Atomic Absorption

  • AA was used to analyze the presence of both iron and gold for all remaining samples


Figure 1. Calibration curve for iron standards Calibration curve of gold standards for atomic absorption analysis of biomineralized proteins with gold and magnetite nanoparticles correct .png
Figure 2. Calibration curve for gold standards Calibration curve of gold standards for atomic absorption analysis of biomineralized proteins with gold and magnetite nanoparticles correct.png
Table 1. Iron Atomic Absorption Analysis of samples

Sample name Concentration Iron in sample (ug/mL) Total concentration Iron originally in solution (ug/mL) Concentration Iron incorporated (ug/mL) Percent Iron incorporated (%)
13500:1 Mag:BSA N2 prep 0.022256152 1765.35 1765.327744 99.99873928
13500:1 Mag:BSA N2 prep with Au 0.022219961 1765.35 1765.32778 99.99874133
13000:1 Mag:BSA oven 0.02224606 1765.35 1765.327754 99.99873985
16500:1 Mag:Heme oven 0.02224084 1765.35 1765.327759 99.99874015
16500:1 Mag:Heme:Au oven 0.022238231 1765.35 1765.327762 99.99874029
16500:1 Mag:Heme:Au sonicated 0.022220831 1765.35 1765.327779 99.99874128
13500:1 Mag:BSA:Au sonicated 0.022207781 1765.35 1765.327792 99.99874202
13000:1 Mag:BSA:Au oven 0.022180812 1765.35 1765.327819 99.99874355


Table 2.Gold atomic absorption analysis of samples

Sample name Concentration Gold in sample (ug/mL) Total concentration Gold originally in solution (ug/mL) Concentration gold incorporated (ug/mL) Percent Gold incorporated (%)
13500:1 Mag:BSA N2 prep with Au 0.04467344 49.2425 49.19782656 99.90927869
13500:1 Mag:BSA:Au sonicated 0.0464768 49.2425 49.1960232 99.90561649
13000:1 Mag:BSA:Au oven 0.04685952 49.2425 49.19564048 99.90483928
16500:1 Mag:Heme:Au oven 0.04447792 49.2425 49.19802208 99.90967575
16500:1 Mag:Heme:Au sonicated 0.04400784 49.2425 49.19849216 99.91063037