User:Moira M. Esson/Notebook/CHEM-571/2014/04/15

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Objectives

  1. Magnetic testing/Fluorescence
  2. Microscope (sonicated and oven samples prepared last week)
  3. Prepare sonicated samples (Au incorporated)


Microscope

  • Please refer to Puja Mody's notebook for all microscope data


Sample preparation

Table 1. FeSO4 stock preparation

Preparation of FeSO4 stock '
Molecular Weight (g/mol) 278.01
mass(g) 0.83403
volume stock (L) 0.01
Concentration (M) 0.3


Table 2. BSA stock preparation

Preparation of BSA stock '
Molecular weight (g/mol) 66463
mass (g) 0.0174
volume stock (L) 0.01
Concentration (M) 2.618E-05


Table 3. Hemoglobin stock

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol) 64500
mass hemglobin (g) 0.0995
volume solution (L) 0.1
Concentration (M) 1.54264E-05


Table 4. KNO3 stock preparation

Preparation of KNO3 '
Molecular Weight (g/mol) 101.1
mass(g) 0.9099
volume stock (L) 0.01
Concentration (M) 0.9


Table 5. KOH stock solution preparation

Preparation of KOH stock '
Molecular Weight (g/mol) 56.11
mass(g) 0.61721
volume stock (L) 0.01
Concentration (M) 1.1


Table 6. Gold stock solution preparation

Preparation gold stock '
Molecular weight Au (g/mol) 393.83
mass Au(g) 0.01446
volume stock(L) 0.01
Concentration (M) 0.003671635


Table 7.Magnetite:BSA preparation

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
13000 2.30769E-06 0.881472149 1 0.909090909 4.444444444 2.764992498


Table 8.Magnetite:Hemoglobin preparation

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
16500 1.81818E-06 1.178620375 1 0.909090909 4.444444444 2.467844272


Table 9.Gold addition to Magnetite:BSA prep

Ratio Concentration BSA Volume BSA Added (mL) Volume Gold (mL) Volume Water(mL)
130 1.92308E-06 0.734560124 0.680895574 8.584544302


Table 10.Gold addition to Magnetite:Hemoglobin prep

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) Volume Gold(mL) Volume Water(mL)
165 1.51515E-06 0.982183646 0.680895574 8.336920781


Atomic Absorption

  • Stock solutions were prepared for AA analysis tomorrow

Table 1. Preparation of Iron standards

Concentration Standard (ug/mL) Added Stock (mL)
5 0.049850449
10 0.099700897
15 0.149551346
20 0.199401795
25 0.249252243


Magnetic Stimulus Testing

General Protocol: