Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2014/04/15"

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(Objectives)
(Sample preparation)
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<br>
 
<br>
 
==Sample preparation==
 
==Sample preparation==
 +
Table 1. FeSO<sub>4</sub> stock preparation
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
 
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
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|}
 
|}
 
<br>
 
<br>
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Table 2. BSA stock preparation
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
 
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
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|}
 
|}
 
<br>
 
<br>
 +
Table 3. Hemoglobin stock
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
 
| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
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|}
 
|}
 
<br>
 
<br>
 +
Table 4. KNO<sub>3</sub> stock preparation
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
 
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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|}
 
|}
 
<br>
 
<br>
 +
Table 5. KOH stock solution preparation
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
 
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
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|}
 
|}
 
<br>
 
<br>
 +
Table 6. Gold stock solution preparation
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
 
| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
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|}
 
|}
 
<br>
 
<br>
Table...Magnetite:BSA preparation
+
Table 7.Magnetite:BSA preparation
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
| align="center" style="background:#f0f0f0;"|'''Ratio'''
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|}
 
|}
 
<br>
 
<br>
Table...Magnetite:Hemoglobin preparation
+
Table 8.Magnetite:Hemoglobin preparation
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Hemoglobin'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Hemoglobin Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 +
|-
 +
| 16500||1.81818E-06||1.178620375||1||0.909090909||4.444444444||2.467844272
 +
|}
 +
<br>
 +
Table 9.Gold addition to Magnetite:BSA prep
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration BSA'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume BSA Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Gold (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Water(mL)'''
 +
|-
 +
| 130||1.92308E-06||0.734560124||0.680895574||8.584544302
 +
|}
 +
<br>
 +
Table 10.Gold addition to Magnetite:Hemoglobin prep
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Hemoglobin'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Hemoglobin Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Gold(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Water(mL)'''
 +
|-
 +
| 165||1.51515E-06||0.982183646||0.680895574||8.336920781
 +
|}
 +
<br>
 +
==Atomic Absorption==
 +
Table 1. Preparation of Iron standards
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Standard (ug/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Added Stock (mL)'''
 +
|-
 +
| 5||0.049850449
 +
|-
 +
| 10||0.099700897
 +
|-
 +
| 15||0.149551346
 +
|-
 +
| 20||0.199401795
 +
|-
 +
| 25||0.249252243
 +
|}
 +
 
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__NOTOC__
 
__NOTOC__

Revision as of 09:39, 29 April 2014

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Objectives

  1. Magnetic testing/Fluorescence
  2. Microscope (sonicated and oven samples prepared last week)
  3. Prepare sonicated samples (Au incorporated)


Microscope

  • Please refer to Puja Mody's notebook for all microscope data


Sample preparation

Table 1. FeSO4 stock preparation

Preparation of FeSO4 stock '
Molecular Weight (g/mol) 278.01
mass(g) 0.83403
volume stock (L) 0.01
Concentration (M) 0.3


Table 2. BSA stock preparation

Preparation of BSA stock '
Molecular weight (g/mol) 66463
mass (g) 0.0174
volume stock (L) 0.01
Concentration (M) 2.618E-05


Table 3. Hemoglobin stock

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol) 64500
mass hemglobin (g) 0.0995
volume solution (L) 0.1
Concentration (M) 1.54264E-05


Table 4. KNO3 stock preparation

Preparation of KNO3 '
Molecular Weight (g/mol) 101.1
mass(g) 0.9099
volume stock (L) 0.01
Concentration (M) 0.9


Table 5. KOH stock solution preparation

Preparation of KOH stock '
Molecular Weight (g/mol) 56.11
mass(g) 0.61721
volume stock (L) 0.01
Concentration (M) 1.1


Table 6. Gold stock solution preparation

Preparation gold stock '
Molecular weight Au (g/mol) 393.83
mass Au(g) 0.01446
volume stock(L) 0.01
Concentration (M) 0.003671635


Table 7.Magnetite:BSA preparation

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
13000 2.30769E-06 0.881472149 1 0.909090909 4.444444444 2.764992498


Table 8.Magnetite:Hemoglobin preparation

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
16500 1.81818E-06 1.178620375 1 0.909090909 4.444444444 2.467844272


Table 9.Gold addition to Magnetite:BSA prep

Ratio Concentration BSA Volume BSA Added (mL) Volume Gold (mL) Volume Water(mL)
130 1.92308E-06 0.734560124 0.680895574 8.584544302


Table 10.Gold addition to Magnetite:Hemoglobin prep

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) Volume Gold(mL) Volume Water(mL)
165 1.51515E-06 0.982183646 0.680895574 8.336920781


Atomic Absorption

Table 1. Preparation of Iron standards

Concentration Standard (ug/mL) Added Stock (mL)
5 0.049850449
10 0.099700897
15 0.149551346
20 0.199401795
25 0.249252243