Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2014/04/01"

From OpenWetWare
Jump to: navigation, search
(→‎Objectives)
(→‎Atomic Absorption)
Line 168: Line 168:
 
| 16000:1 Mag-Heme+ 160:1 Au-Heme||-0.01376067||1765.35||1765.363761||100.0007795
 
| 16000:1 Mag-Heme+ 160:1 Au-Heme||-0.01376067||1765.35||1765.363761||100.0007795
 
|}
 
|}
 +
<br>
 +
==DSC Sample preparation==
 +
General protocol:
 +
#Record the mass of a Tzero pan
 +
#Using a pipette, add ~10μL of sample to the pan(vortex sample prior to removal of 10μL to ensure homogeneous distribution of hydrogels).
 +
#Place pan in fume hood until sample is completely dry.
 +
#Repeat process as necessary (until a large amount of sample is present in the pans)
 +
 +
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 07:12, 29 April 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  1. Schlenk line method sample prep
  2. Fe atomic absorption of samples prepared on 03/18/2014 and 03/19/2014
  3. Samples were prepared for DSC analysis


Schlenk line sample preparation

  • The general protocol for the Schlenk line method of preparation described on 01/29/2014 was followed.
  • For the addition of gold using the Schlenk line method, an equivalent volume of gold:protein samples were prepared, de-gassed using N2, and added to the magnetite reaction mixture using a syringe.

Table 1. BSA stock preparation

Preparation of BSA stock '
Molecular weight (g/mol) 66463
mass (g) 0.0167
volume stock (L) 0.01
Concentration (M) 2.51268E-05


Table 2. Preparation of FeSO4*7H2O stock

Preparation of FeSO4 stock '
Molecular Weight (g/mol) 278.01
mass(g) 0.83403
volume stock (L) 0.01
Concentration (M) 0.3


Table 3. KOH stock

Preparation of KOH stock '
Molecular Weight (g/mol) 56.11
mass(g) 1.5
volume stock (L) 0.025
Concentration (M) 1.069328106


Table 4. Potassium Nitrate stock solution

Preparation of KNO3 '
Molecular Weight (g/mol) 101.1
mass(g) 0.8088
volume stock (L) 0.01
Concentration (M) 0.8


Table 5. Gold stock solution preparation

Preparation gold stock '
Molecular weight Au (g/mol) 393.83
mass Au(g) 0.01446
volume stock(L) 0.01
Concentration (M) 0.003671635


Table 6. Magnetite:Protein solution preparation

Ratio concentration BSA (M) volume of BSA added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
13500 2.22222E-06 0.442202262 0.5 0.467583333 2.5 1.090214405


Table 7. Magnetite:Protein and Gold:Protein solution preparation

Ratio concentration BSA (M) volume of BSA added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
13500 2.22222E-06 0.442202262 0.5 0.467583333 2.5 1.090214405
Ratio Amount of gold added (mL) Concentration BSA (M) volume BSA added (mL) volume water added (mL)
135 0.340447787 1.86124E-07 0.037037037 4.622515176

Atomic Absorption

  • The general protocol for AA was followed. Samples were analyzed for the presence of iron.

Chem572day4-1.png
Table 1. Atomic Absorbance Data for Magnetite:Protein hydrogel samples

Sample number Sample name Abs background actual abs.
1 13000:1 mag- BSA+ 130:1 Au-BSA 0.0117 -0.0005 0.0122
2 13000:1 mag-BSA sonicated 0.0029 0.007 -0.0041
3 13500:1 Mag-BSA + 135:1 Au-BSA 0.0022 -0.0019 0.0041
4 17000:1 Mag-Heme +170:1 Au-Heme 0.0029 -0.0013 0.0042
5 16500:1 Mag-Heme +165:1 Au-Heme 0.002 -0.0021 0.0041
6 16500:1 Mag-Heme sonicator -0.0009 0.0162 -0.0171
7 16000:1 Mag-Heme+ 160:1 Au-Heme 0.0023 0.00066 0.00164


Table 2. Concentration of Iron present in each sample

Sample name Concentration Iron in sample (ug/mL) Total concentration Iron originally in solution (ug/mL) Concentration Iron incorporated (ug/mL) Percent Iron incorporated (%)
13000:1 mag- BSA+ 130:1 Au-BSA -0.01359993 1765.35 1765.3636 100.0007704
13000:1 mag-BSA sonicated -0.01375041 1765.35 1765.36375 100.0007789
13500:1 Mag-BSA + 135:1 Au-BSA -0.01376238 1765.35 1765.363762 100.0007796
17000:1 Mag-Heme +170:1 Au-Heme -0.01375041 1765.35 1765.36375 100.0007789
16500:1 Mag-Heme +165:1 Au-Heme -0.0137658 1765.35 1765.363766 100.0007798
16500:1 Mag-Heme sonicator -0.01381539 1765.35 1765.363815 100.0007826
16000:1 Mag-Heme+ 160:1 Au-Heme -0.01376067 1765.35 1765.363761 100.0007795


DSC Sample preparation

General protocol:

  1. Record the mass of a Tzero pan
  2. Using a pipette, add ~10μL of sample to the pan(vortex sample prior to removal of 10μL to ensure homogeneous distribution of hydrogels).
  3. Place pan in fume hood until sample is completely dry.
  4. Repeat process as necessary (until a large amount of sample is present in the pans)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Moira_M._Esson/Notebook/CHEM-571/2014/04/01&oldid=791380"