Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2014/04/01"

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(Atomic Absorption)
(Schlenk line sample preparation)
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#Fe atomic absorption of samples prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/03/18|03/18/2014]] and [[User:Moira M. Esson/Notebook/CHEM-571/2014/03/19|03/19/2014]]
 
#Fe atomic absorption of samples prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/03/18|03/18/2014]] and [[User:Moira M. Esson/Notebook/CHEM-571/2014/03/19|03/19/2014]]
 
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<br>
==Schlenk line sample preparation==
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==Atomic Absorption==
 
==Atomic Absorption==
 
*The general protocol for AA was followed. Samples were analyzed for the presence of iron.
 
*The general protocol for AA was followed. Samples were analyzed for the presence of iron.

Revision as of 06:00, 29 April 2014

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Objectives

  1. Schlenk line method sample prep
  2. Fe atomic absorption of samples prepared on 03/18/2014 and 03/19/2014



Atomic Absorption

  • The general protocol for AA was followed. Samples were analyzed for the presence of iron.

Chem572day4-1.png
Table 1. Atomic Absorbance Data for Magnetite:Protein hydrogel samples

Sample number Sample name Abs background actual abs.
1 13000:1 mag- BSA+ 130:1 Au-BSA 0.0117 -0.0005 0.0122
2 13000:1 mag-BSA sonicated 0.0029 0.007 -0.0041
3 13500:1 Mag-BSA + 135:1 Au-BSA 0.0022 -0.0019 0.0041
4 17000:1 Mag-Heme +170:1 Au-Heme 0.0029 -0.0013 0.0042
5 16500:1 Mag-Heme +165:1 Au-Heme 0.002 -0.0021 0.0041
6 16500:1 Mag-Heme sonicator -0.0009 0.0162 -0.0171
7 16000:1 Mag-Heme+ 160:1 Au-Heme 0.0023 0.00066 0.00164


Table 2. Concentration of Iron present in each sample

Sample name Concentration Iron in sample (ug/mL) Total concentration Iron originally in solution (ug/mL) Concentration Iron incorporated (ug/mL) Percent Iron incorporated (%)
13000:1 mag- BSA+ 130:1 Au-BSA -0.01359993 1765.35 1765.3636 100.0007704
13000:1 mag-BSA sonicated -0.01375041 1765.35 1765.36375 100.0007789
13500:1 Mag-BSA + 135:1 Au-BSA -0.01376238 1765.35 1765.363762 100.0007796
17000:1 Mag-Heme +170:1 Au-Heme -0.01375041 1765.35 1765.36375 100.0007789
16500:1 Mag-Heme +165:1 Au-Heme -0.0137658 1765.35 1765.363766 100.0007798
16500:1 Mag-Heme sonicator -0.01381539 1765.35 1765.363815 100.0007826
16000:1 Mag-Heme+ 160:1 Au-Heme -0.01376067 1765.35 1765.363761 100.0007795