Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2014/04/01"

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(Atomic Absorption)
(Atomic Absorption)
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  <br>
 
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==Atomic Absorption==
 
==Atomic Absorption==
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*The general protocol for AA was followed. Samples were analyzed for the presence of iron.
 
[[Image:Chem572day4-1.png|500px]]
 
[[Image:Chem572day4-1.png|500px]]
 
+
<br>
 +
Table 1. Atomic Absorbance Data for Magnetite:Protein hydrogel samples
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{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Sample number'''
 +
| align="center" style="background:#f0f0f0;"|'''Sample name'''
 +
| align="center" style="background:#f0f0f0;"|'''Abs'''
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| align="center" style="background:#f0f0f0;"|'''background'''
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| align="center" style="background:#f0f0f0;"|'''actual abs.'''
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|-
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| 1||13000:1 mag- BSA+ 130:1 Au-BSA ||0.0117||-0.0005||0.0122
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|-
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| 2||13000:1 mag-BSA  sonicated||0.0029||0.007||-0.0041
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|-
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| 3||13500:1 Mag-BSA + 135:1 Au-BSA||0.0022||-0.0019||0.0041
 +
|-
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| 4||17000:1 Mag-Heme +170:1 Au-Heme||0.0029||-0.0013||0.0042
 +
|-
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| 5||16500:1 Mag-Heme +165:1 Au-Heme||0.002||-0.0021||0.0041
 +
|-
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| 6||16500:1 Mag-Heme sonicator||-0.0009||0.0162||-0.0171
 +
|-
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| 7||16000:1 Mag-Heme+ 160:1 Au-Heme||0.0023||0.00066||0.00164
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|}
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<br>
 +
Table 2. Concentration of Iron present in each sample
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Sample name'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Iron in sample (ug/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Total concentration Iron originally in solution (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Iron incorporated (ug/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Percent Iron incorporated (%)'''
 +
|-
 +
| 13000:1 mag- BSA+ 130:1 Au-BSA ||-0.01359993||1765.35||1765.3636||100.0007704
 +
|-
 +
| 13000:1 mag-BSA  sonicated||-0.01375041||1765.35||1765.36375||100.0007789
 +
|-
 +
| 13500:1 Mag-BSA + 135:1 Au-BSA||-0.01376238||1765.35||1765.363762||100.0007796
 +
|-
 +
| 17000:1 Mag-Heme +170:1 Au-Heme||-0.01375041||1765.35||1765.36375||100.0007789
 +
|-
 +
| 16500:1 Mag-Heme +165:1 Au-Heme||-0.0137658||1765.35||1765.363766||100.0007798
 +
|-
 +
| 16500:1 Mag-Heme sonicator||-0.01381539||1765.35||1765.363815||100.0007826
 +
|-
 +
| 16000:1 Mag-Heme+ 160:1 Au-Heme||-0.01376067||1765.35||1765.363761||100.0007795
 +
|}
  
 
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Revision as of 06:56, 29 April 2014

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Objectives

  1. Schlenk line method sample prep
  2. Fe atomic absorption of samples prepared on 03/18/2014 and 03/19/2014


Schlenk line sample preparation


Atomic Absorption

  • The general protocol for AA was followed. Samples were analyzed for the presence of iron.

Chem572day4-1.png
Table 1. Atomic Absorbance Data for Magnetite:Protein hydrogel samples

Sample number Sample name Abs background actual abs.
1 13000:1 mag- BSA+ 130:1 Au-BSA 0.0117 -0.0005 0.0122
2 13000:1 mag-BSA sonicated 0.0029 0.007 -0.0041
3 13500:1 Mag-BSA + 135:1 Au-BSA 0.0022 -0.0019 0.0041
4 17000:1 Mag-Heme +170:1 Au-Heme 0.0029 -0.0013 0.0042
5 16500:1 Mag-Heme +165:1 Au-Heme 0.002 -0.0021 0.0041
6 16500:1 Mag-Heme sonicator -0.0009 0.0162 -0.0171
7 16000:1 Mag-Heme+ 160:1 Au-Heme 0.0023 0.00066 0.00164


Table 2. Concentration of Iron present in each sample

Sample name Concentration Iron in sample (ug/mL) Total concentration Iron originally in solution (ug/mL) Concentration Iron incorporated (ug/mL) Percent Iron incorporated (%)
13000:1 mag- BSA+ 130:1 Au-BSA -0.01359993 1765.35 1765.3636 100.0007704
13000:1 mag-BSA sonicated -0.01375041 1765.35 1765.36375 100.0007789
13500:1 Mag-BSA + 135:1 Au-BSA -0.01376238 1765.35 1765.363762 100.0007796
17000:1 Mag-Heme +170:1 Au-Heme -0.01375041 1765.35 1765.36375 100.0007789
16500:1 Mag-Heme +165:1 Au-Heme -0.0137658 1765.35 1765.363766 100.0007798
16500:1 Mag-Heme sonicator -0.01381539 1765.35 1765.363815 100.0007826
16000:1 Mag-Heme+ 160:1 Au-Heme -0.01376067 1765.35 1765.363761 100.0007795