User:Moira M. Esson/Notebook/CHEM-571/2013/11/20: Difference between revisions
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#Analyze invertase-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/19|11/19/2013]] using UV/vis and AA spectroscopy. | #Analyze invertase-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/19|11/19/2013]] using UV/vis and AA spectroscopy. | ||
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==UV/vis spectroscopy== | ==UV/vis spectroscopy== | ||
*The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06|11/06/2013]] was followed. | *The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06|11/06/2013]] was followed. | ||
Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions | Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions | ||
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[[Image:Invertase Absorption Spectra.jpg]] | [[Image:Invertase Absorption Spectra.jpg|1000px]] | ||
==AA spectroscopy== | ==AA spectroscopy== | ||
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Figure 2. Calibration Curve of Gold Standards | Figure 2. Calibration Curve of Gold Standards | ||
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[[Image:Calibration Curve for Gold Standards zem11202013.png]] | [[Image:Calibration Curve for Gold Standards zem11202013.png|800px]] | ||
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Figure 3. Atomic Absorption spectra of Invertase AuNP solutions | Figure 3. Atomic Absorption spectra of Invertase AuNP solutions | ||
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[[Image:Atomic Absorption spectra of Invertase-AuNP solutions zem11202013.png]] | [[Image:Atomic Absorption spectra of Invertase-AuNP solutions zem11202013.png|800px]] | ||
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*The strange shape of this spectra will be investigated. This may be related to the varying color of the solutions. The solutions are colored, then appear colorless, then are again colored (see images below) | *The strange shape of this spectra will be investigated. This may be related to the varying color of the solutions. The solutions are colored, then appear colorless, then are again colored (see images below) | ||
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Figure 4. Absorbance vs. Concentration plot of the Invertase:AuNP solutions | Figure 4. Absorbance vs. Concentration plot of the Invertase:AuNP solutions | ||
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[[Image:Absorbance vs. Concentration plot of Invertase-AuNP solutions zem11202013.png]] | [[Image:Absorbance vs. Concentration plot of Invertase-AuNP solutions zem11202013.png|800px]] | ||
==Notes== | ==Notes== | ||
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Figure 5. BSA-AuNP solutions that did not form fibers. | Figure 5. BSA-AuNP solutions that did not form fibers. | ||
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[[Image:1120131224-1zem11202013.jpg]] | [[Image:1120131224-1zem11202013.jpg|500px]] | ||
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Figure 6. Invertase-AuNP solutions that did form fibers(ratio 30:1 on the left and 50:1 on right). | |||
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[[Image:1120131221zem.jpg|500px]] | |||
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*In comparison to previously prepared fibers, these fibers are much smaller and less string-like in appearance. | |||
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Revision as of 11:09, 10 December 2013
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Objectives
UV/vis spectroscopy
Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions
AA spectroscopy
Notes
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