Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/11/20"

From OpenWetWare
(Objectives)
(UV/vis spectroscopy)
Line 12: Line 12:
 
==UV/vis spectroscopy==
 
==UV/vis spectroscopy==
 
*The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed.
 
*The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed.
 +
Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions
 +
<br>
 +
[[Image:Invertase Absorption Spectra.jpg]]
 +
==AA spectroscopy==
 +
<br>
 +
'''General Protocol'''
 +
#Transfer 3mL of sample from test tubes into a 10mL falcon tube.
 +
#Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
 +
#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]].
 +
#Run samples on AA.
 +
<br>
 +
Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)'''
 +
|-
 +
| 10||0.1||9.9
 +
|-
 +
| 20||0.2||9.8
 +
|-
 +
| 30||0.3||9.7
 +
|-
 +
| 40||0.4||9.6
 +
|-
 +
| 50||0.5||9.5
 +
|}
  
 
==Notes==
 
==Notes==

Revision as of 18:01, 2 December 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  1. Re-prepare BSA-AuNP solutions that were originally prepared on 11/19/2013 because these solutions did not form fibers
  2. Analyze invertase-AuNP solutions prepared on 11/19/2013 using UV/vis and AA spectroscopy.


UV/vis spectroscopy

  • The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed.

Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions
Invertase Absorption Spectra.jpg

AA spectroscopy


General Protocol

  1. Transfer 3mL of sample from test tubes into a 10mL falcon tube.
  2. Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
  3. Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on 09/10/2013.
  4. Run samples on AA.


Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
10 0.1 9.9
20 0.2 9.8
30 0.3 9.7
40 0.4 9.6
50 0.5 9.5

Notes

  • All BSA solutions prepared 11/19/2013 did not form fibers. This did not coincide with all other lab groups data. It is suspected that the BSA stock solution was prepared inaccurately. New nano-particles will be prepared.


Figure 1. BSA-AuNP solutions that did not form fibers.
[[
Figure 2. Invertase-AuNP solutions that did form fibers.
Figure