Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/11/19"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(AuNP solution preparation)
Line 12: Line 12:
 
==AuNP solution preparation==
 
==AuNP solution preparation==
 
*The general protocol for the preparation of AuNPs described on [[User  
 
*The general protocol for the preparation of AuNPs described on [[User  
 
+
<br>
 +
==AA spectroscopy==
 +
<br>
 +
#Transfer 3mL of sample from test tubes into a 10mL falcon tube.
 +
#Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
 +
#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]].
 +
#Run samples on AA.
  
  

Revision as of 11:50, 19 November 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  1. Prepare AuNP-protein solutions for biomineralization
  2. Analyze AuNP-protein solutions prepared on 10/30/2013 using AA spectroscopy.


AuNP solution preparation

  • The general protocol for the preparation of AuNPs described on [[User


AA spectroscopy


  1. Transfer 3mL of sample from test tubes into a 10mL falcon tube.
  2. Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
  3. Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on 09/10/2013.
  4. Run samples on AA.