Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/11/12"

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==UV/vis==
 
==UV/vis==
*The general protocol for UV/vis spectroscopy was  
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*The general protocol for UV/vis spectroscopy described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] was followed.
 +
<br>
 +
Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
 +
<br>
 +
[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions ZEM .png]]
 +
<br>
 +
Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions
 +
<br>
 +
[[Image:Corrected Absorbance Spectra of Hemoglobin-AuNP solutions ZEM .png]]
 +
<br>
 +
 
 +
==Notes==
 +
*All protein-NP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color.
 +
*The solutions with color were(see image below):
 +
**Lysozyme 10:1
 +
**Lysozyme 15:1
 +
**Lysozyme 20:1
 +
**Lysozyme 25:1
 +
**Lysozyme 30:1
 +
**Lysozyme 35:1
 +
**Hemoglobin 30:1
 +
**Hemoglobin 50:1
 +
**Hemoglobin 70:1
 +
**Hemoglobin 90:1
 +
<br>
 +
Figure 3. Lysozyme-AuNP solutions
 +
<br>
 +
[[Image:11122013lysozymezem.jpg]]
 +
*45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right
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Revision as of 08:15, 6 December 2013

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Objectives

  1. Analyze all protein-AuNPs prepared on 11/06/2013 using UV/vis spectroscopy
  2. Prepare standard gold solutions to for analysis of protein-AuNP solutions prepared on 11/06/2013 and 10/30/2013 using AA spectroscopy


UV/vis


Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
Corrected Absorbance Spectra of Lysozyme-AuNP solutions ZEM .png
Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions
Corrected Absorbance Spectra of Hemoglobin-AuNP solutions ZEM .png

Notes

  • All protein-NP solutions prepared on User:Moira M. Esson/Notebook/CHEM-571/2013/11/06 formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color.
  • The solutions with color were(see image below):
    • Lysozyme 10:1
    • Lysozyme 15:1
    • Lysozyme 20:1
    • Lysozyme 25:1
    • Lysozyme 30:1
    • Lysozyme 35:1
    • Hemoglobin 30:1
    • Hemoglobin 50:1
    • Hemoglobin 70:1
    • Hemoglobin 90:1


Figure 3. Lysozyme-AuNP solutions
11122013lysozymezem.jpg

  • 45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right