Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/11/06"

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(UV/vis Analysis)
(UV/vis Analysis)
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Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
 
Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
 
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]]
 
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*Only the 30:1 ratio exhibited an absorbance peak. This was expected as this was the only colored solution or only solution that did not form fibers.
 
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Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
 
Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions

Revision as of 14:46, 11 November 2013

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Entry title

  1. Continue biomineralization. Prepare protein-AuNPs for Hemoglobin and Lysozyme.
  2. Analyze protein-AuNP solutions prepared on 10/30/2013 using UV/vis spectroscopy.


UV/vis Analysis

General Protocol:

  1. Place 1mL of each ratio in an 1.5mL eppendorf tube.
  2. Centrifuge at maximum speed for 30 minutes.
  3. Take a UV-Vis spectrum of each sample from 200nm to 800nm.
  4. After taking the spectrum, store the sample in a fresh eppy tube in an upright position.


Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions

Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png

  • Only the 30:1 ratio exhibited an absorbance peak. This was expected as this was the only colored solution or only solution that did not form fibers.


Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
Corrected Absorbance spectra of Myoglobin Au-NP solution .png

Protein-AuNP solution preparation

  • The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.
  • 5mL of each solution was prepared.
  • All solutions were prepared using deionized H2O
  • Tri-hydrate gold was used for the preparation of the gold standard solution.
  • All stock solutions were prepared in a 10mL volumetric flask.


Table 1. Preparation of Hemoglobin Stock solution

Molecular weight (g/mol) 64500
mass myoglobin (g) 0.0106
volume solution (L) 0.01
Concentration (M) 1.64341E-05


Table 2. Preparation of Gold stock solution

Molecular weight Au (g/mol) 393.83
mass Au(g) 0.032
volume stock(L) 0.025
Concentration (M) 0.003250133


Table 3. Preparation of Lysozyme stock solution

Molecular weight (g/mol) 14307
mass lysozyme (g) 0.0717
volume solution (L) 0.05
Concentration (M) 0.00010028


Table 4. Preparation of Hemoglobin-AuNP solutions

Ratio Amount of gold added concentration hemoglobin (M) volume Hemoglobin (mL) Volume Water (mL)
30 0.384599609 8.33333E-06 2.535377358 2.080023032
50 0.384599609 0.000005 1.521226415 3.094173976
70 0.384599609 3.57143E-06 1.086590296 3.528810094
90 0.384599609 2.77778E-06 0.845125786 3.770274604
110 0.384599609 2.27273E-06 0.691466552 3.923933838
130 0.384599609 1.92308E-06 0.585087083 4.030313308
150 0.384599609 1.66667E-06 0.507075472 4.108324919
170 0.384599609 1.47059E-06 0.447419534 4.167980857
190 0.384599609 1.31579E-06 0.400322741 4.21507765
210 0.384599609 1.19048E-06 0.362196765 4.253203625


Table 5. Preparation of Lysozyme Au-NP solutions

Ratio Amount gold added (mL) Concentration Lysozyme (M) Volume lysozyme (mL) Volume water (mL)
5 0.384599609 0.00005 2.493019545 2.122380845
10 0.384599609 0.000025 1.246509773 3.368890618
15 0.384599609 1.66667E-05 0.831006515 3.784393876
20 0.384599609 0.0000125 0.623254886 3.992145504
25 0.384599609 0.00001 0.498603909 4.116796482
30 0.384599609 8.33333E-06 0.415503258 4.199897133
35 0.384599609 7.14286E-06 0.356145649 4.259254741
40 0.384599609 0.00000625 0.311627443 4.303772947
45 0.384599609 5.55556E-06 0.277002172 4.338398219
50 0.384599609 0.000005 0.249301955 4.366098436
  • New ratios of lysozyme were prepared in order to determine the optimal concentration ratios for the formation of fibers.