Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/10/23"

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(Objectives)
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#Synthesize new HRP-AuNP solutions and Myoglobin-AuNP solutions for future catalytic activity testing.
 
#Synthesize new HRP-AuNP solutions and Myoglobin-AuNP solutions for future catalytic activity testing.
 
<br>
 
<br>
==
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==HRP-AuNP and Myoglobin-AuNP==
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*The protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/22|10/22/2013]] did not produce fibers. As such, new protein-AuNP solutions with increased ratios of protein-AuNP were prepared in order to determine the optimal concentration of Au to protein to synthesize fibers.
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*The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]].
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Table 1. Stock solutions used in the preparation of Protein-AuNP solutions
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Concentration HRP Day 2(M)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Gold Stock (M)'''
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| align="center" style="background:#f0f0f0;"|'''Myoglobin (M)'''
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|-
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| 0.0000015||0.0025||0.0000165
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|}
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Table 2. Preparation of Protein-AuNP solutions
  
  

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Objectives

  1. Run UV-vis on the 20 protein-Au nanoparticle samples prepared on 10/22/2013.
  2. Synthesize new HRP-AuNP solutions and Myoglobin-AuNP solutions for future catalytic activity testing.


HRP-AuNP and Myoglobin-AuNP

  • The protein-AuNP solutions prepared on 10/22/2013 did not produce fibers. As such, new protein-AuNP solutions with increased ratios of protein-AuNP were prepared in order to determine the optimal concentration of Au to protein to synthesize fibers.
  • The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.


Table 1. Stock solutions used in the preparation of Protein-AuNP solutions

Concentration HRP Day 2(M) Concentration Gold Stock (M) Myoglobin (M)
0.0000015 0.0025 0.0000165

Table 2. Preparation of Protein-AuNP solutions