Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/10/22"

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(Objectives)
(HRP-AuNP and Pepsin-AuNP)
Line 16: Line 16:
 
{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of gold added'''
 
| align="center" style="background:#f0f0f0;"|'''concentration HRP(M)'''
 
| align="center" style="background:#f0f0f0;"|'''concentration HRP(M)'''
 
| align="center" style="background:#f0f0f0;"|'''concentration Pepsin(M)'''
 
| align="center" style="background:#f0f0f0;"|'''concentration Pepsin(M)'''
Line 23: Line 24:
 
| align="center" style="background:#f0f0f0;"|'''Volume Water Pepsin (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Water Pepsin (mL)'''
 
|-
 
|-
| 30||8.33333E-06||8.33333E-06||2.723311547||2.653927813||1.780656707||1.850040441
+
| 30||0.496031746||8.33333E-06||8.33333E-06||2.723311547||2.653927813||1.780656707||1.850040441
 
|-
 
|-
| 50||0.000005||0.000005||1.633986928||1.592356688||2.869981326||2.911611566
+
| 50||0.496031746||0.000005||0.000005||1.633986928||1.592356688||2.869981326||2.911611566
 
|-
 
|-
| 70||3.57143E-06||3.57143E-06||1.16713352||1.137397634||3.336834734||3.36657062
+
| 70||0.496031746||3.57143E-06||3.57143E-06||1.16713352||1.137397634||3.336834734||3.36657062
 
|-
 
|-
| 90||2.77778E-06||2.77778E-06||0.907770516||0.884642604||3.596197738||3.61932565
+
| 90||0.496031746||2.77778E-06||2.77778E-06||0.907770516||0.884642604||3.596197738||3.61932565
 
|-
 
|-
| 110||2.27273E-06||2.27273E-06||0.742721331||0.723798494||3.761246923||3.780169759
+
| 110||0.496031746||2.27273E-06||2.27273E-06||0.742721331||0.723798494||3.761246923||3.780169759
 
|-
 
|-
| 130||1.92308E-06||1.92308E-06||0.628456511||0.61244488||3.875511743||3.891523374
+
| 130||0.496031746||1.92308E-06||1.92308E-06||0.628456511||0.61244488||3.875511743||3.891523374
 
|-
 
|-
| 150||1.66667E-06||1.66667E-06||0.544662309||0.530785563||3.959305945||3.973182691
+
| 150||0.496031746||1.66667E-06||1.66667E-06||0.544662309||0.530785563||3.959305945||3.973182691
 
|-
 
|-
| 170||1.47059E-06||1.47059E-06||0.480584391||0.468340202||4.023383863||4.035628052
+
| 170||0.496031746||1.47059E-06||1.47059E-06||0.480584391||0.468340202||4.023383863||4.035628052
 
|-
 
|-
| 190||1.31579E-06||1.31579E-06||0.42999656||0.419041234||4.073971694||4.08492702
+
| 190||0.496031746||1.31579E-06||1.31579E-06||0.42999656||0.419041234||4.073971694||4.08492702
 
|-
 
|-
| 210||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709
+
| 210||0.496031746||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709
 
|}
 
|}
  

Revision as of 09:33, 22 October 2013

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Objectives

  1. Determine the absorbance of BSA-AuNPs synthesized on 10/16/2013.


HRP-AuNP and Pepsin-AuNP


Table 1. Preparation of Protein-AuNP solutions

Ratio Amount of gold added concentration HRP(M) concentration Pepsin(M) Volume HRP(mL) Volume Pepsin(mL) Volume Water HRP (mL) Volume Water Pepsin (mL)
30 0.496031746 8.33333E-06 8.33333E-06 2.723311547 2.653927813 1.780656707 1.850040441
50 0.496031746 0.000005 0.000005 1.633986928 1.592356688 2.869981326 2.911611566
70 0.496031746 3.57143E-06 3.57143E-06 1.16713352 1.137397634 3.336834734 3.36657062
90 0.496031746 2.77778E-06 2.77778E-06 0.907770516 0.884642604 3.596197738 3.61932565
110 0.496031746 2.27273E-06 2.27273E-06 0.742721331 0.723798494 3.761246923 3.780169759
130 0.496031746 1.92308E-06 1.92308E-06 0.628456511 0.61244488 3.875511743 3.891523374
150 0.496031746 1.66667E-06 1.66667E-06 0.544662309 0.530785563 3.959305945 3.973182691
170 0.496031746 1.47059E-06 1.47059E-06 0.480584391 0.468340202 4.023383863 4.035628052
190 0.496031746 1.31579E-06 1.31579E-06 0.42999656 0.419041234 4.073971694 4.08492702
210 0.496031746 1.19048E-06 1.19048E-06 0.389044507 0.379132545 4.114923747 4.124835709