Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/10/09"

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(Entry title)
(Kinetics)
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*A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
 
*A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
 
*2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
 
*2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
*A more detailed protocol    
+
*A more detailed protocol is described in [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]] notebook
 +
<br>
 +
Prepared solutions:
 +
*Note: The following solution was prepared by [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]]
 +
<br>
 +
EHNA stock
 +
5mg EHNA in 1mL DMSO ---> 15.9mM EHNA
 +
(1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA
 +
The reaction samples will contain roughly 1nM EHNA
 +
<br>
 +
'''UV/vis'''     
  
 
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Revision as of 17:57, 12 October 2013

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Objectives

  1. Measure the kinetics of the conversion of adenosine to inosine using ADA as a catalyst, in the presence of the inhibitor EHNA.


Kinetics

General Protocol:

  • The general protocol followed the general protocol described on 2013/10/08.
  • A new solution of 40μM adenosine solution was prepared following the protocol described on 2013/10/08.
  • 2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
  • A more detailed protocol is described in Matt Harting's notebook


Prepared solutions:


EHNA stock 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA (1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA The reaction samples will contain roughly 1nM EHNA
UV/vis