User:Moira M. Esson/Notebook/CHEM-571/2013/10/09: Difference between revisions

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(Autocreate 2013/10/09 Entry for User:Moira_M._Esson/Notebook/CHEM-571)
 
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==Entry title==
==Objectives==
* Insert content here...
# Measure the kinetics of the conversion of adenosine to inosine using ADA as a catalyst, in the presence of the inhibitor EHNA.
 
<br>
==Kinetics==
'''General Protocol''':
*The general protocol followed the general protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
*A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
*2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
*A more detailed protocol   


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Revision as of 18:53, 12 October 2013

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Objectives

  1. Measure the kinetics of the conversion of adenosine to inosine using ADA as a catalyst, in the presence of the inhibitor EHNA.


Kinetics

General Protocol:

  • The general protocol followed the general protocol described on 2013/10/08.
  • A new solution of 40μM adenosine solution was prepared following the protocol described on 2013/10/08.
  • 2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
  • A more detailed protocol