Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/10/09"

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(Autocreate 2013/10/09 Entry for User:Moira_M._Esson/Notebook/CHEM-571)
 
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==Entry title==
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==Objectives==
* Insert content here...
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# Measure the kinetics of the conversion of adenosine to inosine using ADA as a catalyst, in the presence of the inhibitor EHNA.
 
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<br>
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==Kinetics==
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'''General Protocol''':
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*The general protocol followed the general protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
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*A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
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*2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
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*A more detailed protocol   
  
 
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Revision as of 18:53, 12 October 2013

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Objectives

  1. Measure the kinetics of the conversion of adenosine to inosine using ADA as a catalyst, in the presence of the inhibitor EHNA.


Kinetics

General Protocol:

  • The general protocol followed the general protocol described on 2013/10/08.
  • A new solution of 40μM adenosine solution was prepared following the protocol described on 2013/10/08.
  • 2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
  • A more detailed protocol