User:Moira M. Esson/Notebook/CHEM-571/2013/10/01: Difference between revisions
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#Buffer: 5mM Tris pH=8 | #Buffer: 5mM Tris pH=8 | ||
#HRP: 1.7μM HRP in 50mL 5mM Tris pH=8 | #HRP: 1.7μM HRP in 50mL 5mM Tris pH=8 | ||
*Performed 20 | *Performed 1/20 dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution). | ||
**Make a 10mL stock solution of 20 | **Make a 10mL stock solution of 1/20 dilute luminol stock solution. | ||
*Prepared stock solution of hydrogen peroxide was too dilute to measure (characteristic peak at 432 nm was not present) | |||
*Reaction did not occur because of the low concentration of hydrogen peroxide | |||
Revision as of 11:19, 1 October 2013
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Objectives
UV-visStock solutions prepared before class:
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