User:Moira M. Esson/Notebook/CHEM-571/2013/09/18: Difference between revisions

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#Take UV/vis sample at the minimum redox potential value.
#Take UV/vis sample at the minimum redox potential value.


at 750 the data should be flat. (subtracted the average from around 750 from the negative values)


 
normalize the data for concentration(divide absorbance by concentration)
figure out what percentage something is oxidized or reduced(shifted to higher wavelengths)
percent oxidized= (abs.(max wavelength) partially oxidized-abs.(max wavelength)fully reduced)/(Abs.(max wavelength)fully oxidized-Abs.(wavlength)fully reduced)
*All of these should be the same wavelength!!!
plot percent oxidized/reduced versus cell potential
for cell potential use the minimum
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Revision as of 09:28, 23 September 2013

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Objectives

  1. Perform redox titration on HRP to determine the standard redox potential of the protein
  • Note: Dr. Hartings the course instructor performed the reduction of HRP, which was unsuccessful on 09/17/2013 prior to the lab. All info can be found here.
  1. Take UV/vis sample at the minimum redox potential value.

at 750 the data should be flat. (subtracted the average from around 750 from the negative values)

normalize the data for concentration(divide absorbance by concentration) figure out what percentage something is oxidized or reduced(shifted to higher wavelengths) percent oxidized= (abs.(max wavelength) partially oxidized-abs.(max wavelength)fully reduced)/(Abs.(max wavelength)fully oxidized-Abs.(wavlength)fully reduced)

  • All of these should be the same wavelength!!!

plot percent oxidized/reduced versus cell potential for cell potential use the minimum


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