Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/09/17"

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(Autocreate 2013/09/17 Entry for User:Moira_M._Esson/Notebook/CHEM-571)
 
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==Entry title==
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==Objectives==
* Insert content here...
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* Find endpoints of HRP (sodium dithionite will reduce and potassium ferrocyanide will oxidize).All buffers are degassed (bubbled nitrogen through for 3 hours)---endpoints of oxidation and reduction of HRP. look for 100% oxidized and 100% reduced.
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*Add very carefully and very slowly and then just let it sit for about 5 minutes before take the sample. Need blank spectra(buffer) of the cuvette
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*solutions HRP that are 1mL 10μM when have 17.25μM starting.  
  
  

Revision as of 08:21, 17 September 2013

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Objectives

  • Find endpoints of HRP (sodium dithionite will reduce and potassium ferrocyanide will oxidize).All buffers are degassed (bubbled nitrogen through for 3 hours)---endpoints of oxidation and reduction of HRP. look for 100% oxidized and 100% reduced.
  • Add very carefully and very slowly and then just let it sit for about 5 minutes before take the sample. Need blank spectra(buffer) of the cuvette
  • solutions HRP that are 1mL 10μM when have 17.25μM starting.