User:Mobeen Ashraf/Notebook/Chem-581/2012/11/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 22: Line 22:
==Description==
==Description==


The following sulfate/porphyrin solutions were prepared and the absorbance was measured for each one between 200 and 800 nm by using UV/VIS instrument:
Sulfate (SO4 2-) as generated by the following solutions along with the porphyrin solution were prepared. The absorbance was measured for each one between by using the UV-Vis instrument by Shimadzu and the selected wavelength range in nm was [200 nm- 800 nm]:


1) 1mL 50μM Na2SO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL of 50μM Na2SO4 was dissolved in a 1mL of 5μM porphyrin solution and 2 mL HPLC graded water was used


2) 1mL 50μM Fe2(SO4)3, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL of 50μM Fe2(SO4)3 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used


3) 1mL 50μM FeSO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL of 50μM FeSO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used


4) 1mL 50μM H2SO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL 50μM H2SO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used


5) 1mL 50μM Al2(SO4)3
* 1mL 50μM Al2(SO4)3 was also dissolved in a 1mL of a porphyrin solution and 2 mL HPLC graded water was used.


==Data==
==Data==

Revision as of 16:18, 30 November 2012

Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

  • Make 1:1 ratio of sulfate ions solution ( H2SO4, Fe2(SO4)3, FeSO4, Na2SO4, Al2(SO4)3).
  • Adsorption through films similar to clay films instead of clay :
    • 1.04 g Fe(II) Cl, 0.5 g Iron Sulfate, 1 film, and 40 ml H2O.
    • To precipitate iron oxide out of the solution NaOH ( 2.67 g in 10 ml H2O) was added drop wise with stirring.
    • The solution was then filtered using vacuum filtration and left to dry.
  • Add film with Porphyrin and glutaraldehyde to 1% solution of HCl and leave out until the next working day.

Description

Sulfate (SO4 2-) as generated by the following solutions along with the porphyrin solution were prepared. The absorbance was measured for each one between by using the UV-Vis instrument by Shimadzu and the selected wavelength range in nm was [200 nm- 800 nm]:

  • 1mL of 50μM Na2SO4 was dissolved in a 1mL of 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL of 50μM Fe2(SO4)3 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL of 50μM FeSO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL 50μM H2SO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL 50μM Al2(SO4)3 was also dissolved in a 1mL of a porphyrin solution and 2 mL HPLC graded water was used.

Data

Notes

Note: The graph in the data section is a courtesy of Allison Alix.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Mobeen_Ashraf/Notebook/Chem-581/2012/11/02&oldid=661787"