User:Mobeen Ashraf/Notebook/Chem-581/2012/10/24

Experimental Chemistry

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Objective Objective 1) Porphyrin Cross-Linking 9again) with 2 drops of H2SO4 and cross link with out Na2SO4. Objective 2) Perform Porphyrin dilutions Objective 3) UV-Vis analysis of porphyrin dilutions. Description Porphyrin cross-linking was done again by placing 2 drops of H2SO4 and it was cross-linked with out Na2SO4. To do that, 2 drops of HCl, 1 ml of glutaraldeyde, ml of porphyrin was used. UV-VIS of Porphyrin Solutions: 100 micro molar, 50 micro molar, 25 micro molar, 12.5 micro molar, and 6.75 micro molar porphyrin dilutions were made for UV-VIS analysis. 3.375 micro molar and 1.6875 micro molar solution of porphyrin solutions were made. 3 stock solutions of 50 micro molar sodium sulfate (Na2SO4), Iron (II) sulfate (FeSO4) and aluminium sulfate (Al2(SO4)3) were prepared. 50 micro molar solution for Na2SO4 was prepared in a following stoichiometric way: Molecular Weight= 142.07g/mol (142.07g/mol)* ( 50 μ mol/1L)* (0.000001mol/1μ mol)* (1L/1,000 ml) * 10 ml = 0.071035 g in 10 ml of HPLC graded water. We lowered the concentration down to 50 m M (milli molar) in a following way: (50 mM) V1= (50 μΜ) (10 mL) V1=0.01 mL. Therefore, 10 μL in 9.99 ml (999 μL) was added to make 50 mM Na2SO4 solution. Note: The same procedure was employed when making 50μM and 50 mM solutions of FeSO4 and Al2(SO4)3. 0.144 g of Fe2(SO4)3 was added in 10 ml of HPLC water to make 50 mM solution and 0.328 g of Al2(SO4)3 was added in 10 ml of HPLC graded water to make 50 mM solution. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.