Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2013/02/27"

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*10mM Quercetin stock was prepared.
 
*10mM Quercetin stock was prepared.

Revision as of 10:48, 5 March 2013

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Procedure

  • Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
    • The following concentrations were used.
Concentration [Ado] (uM) Velocity (nm/sec) 1/[Ado] (1/uM) 1/velocity (sec/nm) ' (-1)/Km -0.022385428
10.52 3.70166E-05 0.095057034 27014.92537 Km 44.67191792
12.34 5.13812E-05 0.081037277 19462.36559 1/Vmax 2662.131337
14.93 6.0221E-05 0.066979236 16605.50459 Vmax 0.000375639
18.87 7.45856E-05 0.052994171 13407.40741
25.64 8.67403E-05 0.03900156 11528.66242
40 9.94475E-05 0.025 10055.55556


  • 10mM Quercetin stock was prepared.
  • Cuvettes with the following solutions were prepared and analyzed.
    • Today, we only had time to complete the first two.
[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Volume Inhibitor Stock solution added [Inhibitor Stock Solution] (uM) [Final Inhibitor] (uM) Total Volume (mL)
0.5 0.02 0.00333 52.63157895 0.6 10.52631579 1.78 0.6 65 13 3
0.5 0.02 0.00333 61.72839506 0.6 12.34567901 1.78 0.6 65 13 3
0.5 0.02 0.00333 74.62686567 0.6 14.92537313 1.78 0.6 65 13 3
0.5 0.02 0.00333 94.33962264 0.6 18.86792453 1.78 0.6 65 13 3
0.5 0.02 0.00333 128.2051282 0.6 25.64102564 1.78 0.6 65 13 3
0.5 0.02 0.00333 200 0.6 40 1.78 0.6 65 13 3

Data

  • The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.

3 5data.JPG

  • Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.