User:Michael F. Nagle/Notebook/Chem 571/2013/02/13: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 10: Line 10:




==Data==
* All Graphs and Tables are for the Combined Data:


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Final Concentration Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''Average velocity over 180 seconds (nm/sec)'''
| align="center" style="background:#f0f0f0;"|'''1/[Adenosine] (1/uM)'''
| align="center" style="background:#f0f0f0;"|'''1/average velocity (sec/nm)'''
|-
| 200||0.000180385||0.005||5543.691027
|-
| 150||0.000183794||0.006666667||5440.881764
|-
| 100||0.000162678||0.01||6147.11077
|-
| 40||0.000111981||0.025||8930.093256
|-
| 16||7.95434E-05||0.0625||12571.75005
|-
| 6.4||3.42451E-05||0.15625||29201.27683
|}
[[Image:Combined_Average_Velocity_over_COncentration.png]]
[[Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png]]
* Km calculations
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Equation'''
| align="center" style="background:#f0f0f0;"|'''Value calculated'''
|-
| (-1)/Km||0.028053516
|-
| Km||35.64615572
|-
| 1/Vmax||4440.372695
|-
| Vmax||0.000225206
|}


==Notes==
==Notes==

Revision as of 14:27, 8 May 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>




Notes

  • For run #2 concentration 40 uM was removed as an outlier
  • For run #1 no values for concentration 150 uM were used
  • Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid Ki value)
Myricetin 32 uM
Kaempferol 32 uM
Quericetin 26 uM



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2013/02/13&oldid=697164"