User:Michael F. Nagle/Notebook/Chem 571/2013/02/13: Difference between revisions
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== | ==Objective== | ||
* | Use data collected yesterday to calculate Average values of the Assay and calculate Km | ||
==Description== | |||
*Calculations using all three assays were performed: | |||
# Velocity by Concentration was graphed for each run | |||
# An average velocity of all three runs was graphed by concentration | |||
# A Lineweaver-Burke Plot was graphed for each run and Km was calculated | |||
# A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated | |||
# Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values | |||
*Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs | |||
# An Average velocity of combine data was graphed by concentration | |||
# A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated | |||
# Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values | |||
==Data== | |||
* All Graphs and Tables are for the Combined Data: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Final Concentration Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Average velocity over 180 seconds (nm/sec)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/[Adenosine] (1/uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/average velocity (sec/nm)''' | |||
|- | |||
| 200||0.000180385||0.005||5543.691027 | |||
|- | |||
| 150||0.000183794||0.006666667||5440.881764 | |||
|- | |||
| 100||0.000162678||0.01||6147.11077 | |||
|- | |||
| 40||0.000111981||0.025||8930.093256 | |||
|- | |||
| 16||7.95434E-05||0.0625||12571.75005 | |||
|- | |||
| 6.4||3.42451E-05||0.15625||29201.27683 | |||
|} | |||
[[Image:Combined_Average_Velocity_over_COncentration.png]] | |||
[[Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png]] | |||
* Km calculations | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Equation''' | |||
| align="center" style="background:#f0f0f0;"|'''Value calculated''' | |||
|- | |||
| (-1)/Km||0.028053516 | |||
|- | |||
| Km||35.64615572 | |||
|- | |||
| 1/Vmax||4440.372695 | |||
|- | |||
| Vmax||0.000225206 | |||
|} | |||
==Notes== | |||
* For run #2 concentration 40 uM was removed as an outlier | |||
* For run #1 no values for concentration 150 uM were used | |||
* Ki values for the inhibitors were found in a paper from Planta Medica: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Flavanoid''' | |||
| align="center" style="background:#f0f0f0;"|'''Ki value)''' | |||
|- | |||
| Myricetin||32 uM | |||
|- | |||
| Kaempferol||32 uM | |||
|- | |||
| Quericetin||26 uM | |||
|} | |||
Revision as of 18:27, 21 February 2013
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ObjectiveUse data collected yesterday to calculate Average values of the Assay and calculate Km Description
Data
Notes
|