Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2013/02/12"

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(Autocreate 2013/02/12 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
 
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| colspan="2"|
 
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==Entry title==
+
==Objective==
* Insert content here...
+
Repeat ADA Assay 2 times for additional data
 +
 
 +
==Description==
 +
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
 +
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 +
**Dilution Tables are located below
 +
 
 +
==Concentration Tables==
 +
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 +
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 +
|-
 +
| 5000||200||800||1000||1
 +
|-
 +
| 5000||150||850||750||1
 +
|-
 +
| 5000||100||900||500||1
 +
|-
 +
| 5000||40||960||200||1
 +
|-
 +
| 5000||16||984||80||1
 +
|-
 +
| 5000||6.4||993.6||32||1
 +
|}
 +
 
 +
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 +
|-
 +
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
 +
|-
 +
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
 +
|}
 +
 
 +
==Data==
 +
Run#1
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 +
|-
 +
| 200||0.000116022
 +
|-
 +
| 150||0.000192818
 +
|-
 +
| 100||0.000139227
 +
|-
 +
| 40||5.55556E-06
 +
|-
 +
| 16||6.68508E-05
 +
|-
 +
| 6.4||0.000039
 +
|}
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 +
|-
 +
| 0.005||8619.047619
 +
|-
 +
| 0.006666667||5186.246418
 +
|-
 +
| 0.01||7182.539683
 +
|-
 +
| 0.025||180000
 +
|-
 +
| 0.0625||14958.67769
 +
|-
 +
| 0.15625||25641.02564
 +
|}
 +
 
 +
 
 +
Run#2
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 +
|-
 +
| 200||0.00021989
 +
|-
 +
| 150||0.000147514
 +
|-
 +
| 100||0.000144751
 +
|-
 +
| 40||0.00010884
 +
|-
 +
| 16||8.12155E-05
 +
|-
 +
| 6.4||3.8674E-05
 +
|}
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 +
|-
 +
| 0.005||4547.738693
 +
|-
 +
| 0.006666667||6779.026217
 +
|-
 +
| 0.01||6908.396947
 +
|-
 +
| 0.025||9187.817259
 +
|-
 +
| 0.0625||12312.92517
 +
|-
 +
| 0.15625||25857.14286
 +
|}
  
  

Revision as of 17:27, 21 February 2013

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Objective

Repeat ADA Assay 2 times for additional data

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • Dilution Tables are located below

Concentration Tables

This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
5000 200 800 1000 1
5000 150 850 750 1
5000 100 900 500 1
5000 40 960 200 1
5000 16 984 80 1
5000 6.4 993.6 32 1

This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.

[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (mL) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Total Volume (mL)
0.5 0.02 0.00333 1000 0.6 200 2.38 3
0.5 0.02 0.00333 750 0.6 150 2.38 3
0.5 0.02 0.00333 500 0.6 100 2.38 3
0.5 0.02 0.00333 200 0.6 40 2.38 3
0.5 0.02 0.00333 80 0.6 16 2.38 3
0.5 0.02 0.00333 32 0.6 6.4 2.38 3

Data

Run#1

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.000116022
150 0.000192818
100 0.000139227
40 5.55556E-06
16 6.68508E-05
6.4 0.000039
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 8619.047619
0.006666667 5186.246418
0.01 7182.539683
0.025 180000
0.0625 14958.67769
0.15625 25641.02564


Run#2

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.00021989
150 0.000147514
100 0.000144751
40 0.00010884
16 8.12155E-05
6.4 3.8674E-05
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 4547.738693
0.006666667 6779.026217
0.01 6908.396947
0.025 9187.817259
0.0625 12312.92517
0.15625 25857.14286