Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2013/01/30"

From OpenWetWare
Jump to: navigation, search
(Preparation of adenosine stock solution)
(Preparation of ADA aliquots)
(One intermediate revision by the same user not shown)
Line 10: Line 10:
==Preparation of phosphate buffer==
*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
*pH was brought to 7.4 using NaOH
==Preparation of ADA aliquots==
*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] measured out 1.7g 0.5unit/mL ADA
*Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.

Revision as of 14:22, 8 May 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>