User:Michael F. Nagle/Notebook/Chem 571/2013/01/30: Difference between revisions
From OpenWetWare
No edit summary |
|||
(One intermediate revision by the same user not shown) | |||
Line 23: | Line 23: | ||
==Preparation of phosphate buffer== | ==Preparation of phosphate buffer== | ||
*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken. | *3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken. | ||
*pH was brought to 7.4 NaOH | *pH was brought to 7.4 using NaOH | ||
==Preparation of ADA aliquots== | ==Preparation of ADA aliquots== | ||
*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] | *[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] measured out 1.7g 0.5unit/mL ADA | ||
*Stock solution was prepared by adding 68 mL phosphate buffer to the ADA | |||
*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer. | *This was divided into 68 1mL centrifuge tubes, which were stored in the freezer. | ||
Revision as of 12:58, 30 January 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Preparation of adenosine stock solution
Preparation of phosphate buffer
Preparation of ADA aliquots
|