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(Preparation of phosphate buffer)
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==Objective==
 
*Prepare stock solutions of adenosine and phosphate buffer
 
*Prepare aliquots of ADA stock solution
 
**Stocks will be used for ADA activity assay next week
 
  
==Preparation of adenosine stock solution==
 
*10mL of 15mM solution of adenosine was prepared
 
** .015M = x/.01
 
**0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
 
**401mg adenosine was weighed and added to 10mL phosphate buffer.
 
*The 15mM solution was diluted to 5mM
 
**.01L * .015M = .005M * x
 
**0.03L total volume for 5mM
 
**0.02L buffer was added to bring the solution to 30mL and 5mM
 
  
==Preparation of phosphate buffer==
 
*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
 
*pH was brought to 7.4 using NaOH
 
  
==Preparation of ADA aliquots==
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*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] measured out 1.7g 0.5unit/mL ADA
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*Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
 
*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.
 
  
  

Latest revision as of 21:23, 26 September 2017

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