User:Michael F. Nagle/Notebook/Chem 571/2012/11/07

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Bradford Assay

A standard curve was obtained with the following concentrations of BSA:

[table] [tr] [th]volume BSA (uL)[/th] [th]volume H2O (uL)[/th] [th]concentration BSA (mg/mL)[/th] [th]concentration BSA (molarity)[/th] [/tr] [tr] [td]0[/td] [td]30[/td] [td]0[/td] [td]0.00E+00[/td] [/tr] [tr] [td]5[/td] [td]25[/td] [td].24[/td] [td]3.61E-06[/td] [/tr] [tr] [td]10[/td] [td]20[/td] [td].15[/td] [td]2.26E-06[/td] [/tr] [tr] [td]15[/td] [td]15[/td] [td].13[/td] [td]2.01E-06[/td] [/tr] [tr] [td]20[/td] [td]10[/td] [td].15[/td] [td]2.26E-06[/td] [/tr] [tr] [td]25[/td] [td]5[/td] [td].24[/td] [td]3.61E-06[/td] [/tr] [tr] [td]30[/td] [td]0[/td] [td]1[/td] [td]1.51E-05[/td] [/tr] [/table]

  • The ADA made Oct. 23 was analyzed.
  • The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.